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The process of introducing nucleic acids into eukaryotic cells by nonviral methods is defined as transfection. Using various chemical, lipid or physical methods, this gene transfer technology is a powerful tool to study gene function and protein expression in the context of a cell. This presentation will provide an overview of the techniques used for transfection along with best practices in setting up and optimizing a transfection system with particular focus to using the FuGENE® HD reagent.

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