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Cell-free expression is a convenient method for rapidly producing protein. Coupled transcription-translation systems provide the advantage of using plasmid DNA as the temple for cell-free expression with no need to first transcript the DNA. The researcher not only has flexibility in which template to use, but also in which system to use as there are now mammalian, plant, bacterial and insect cells systems. Protein detection is a requirement for most applications. Traditionally, one would add radioactive [35S]-methionine to their cell-free expression reactions. With increased regulations on radioactivity, researchers are now using other detection methods such as Fluorotech, Transcend, and Western blotting. Researchers are using cell-free expressed proteins in many applications including protein:protein pulldowns, protein:nucleuic acid shift assays, and enzymatic assays. With new systems that can produce large amounts of protein, researchers are expanding this list of applications.

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