Promega Corporation




Promega @cademy


Homepage

Previous Webinars

Other Tools

Contact

Ask a Scientist

Ask Customer Service

Promega @cademy: Recording/Presentation Request Form

Information

Incomplete solubilization and digestion, and poor peptide recovery are frequent problems in protein sample preparation for mass spectrometry analysis. Additives such as SDS or urea can improve protein solubilization and denaturation but they tend to have a negative effect on digestion and mass spec analysis. To overcome these problems we recently developed a mass spectrometry compatible surfactant sodium 3-((1-(furan-2yl)undecyloxy)carbonylamino) propane -1-sulfonate (ProteaseMAX®). We will show that the surfactant improves protein solubilization, digestion and peptide recovery while avoiding negative effects observed with common solubilizers. We will demonstrate how the positive effects of ProteaseMAX lead to an increase in the number of identified proteins and in protein coverage. The examples will include in-solution and in-gel protein digestion studies performed by our customers. We are aware of a general concern regarding the use of surfactants in LC-MS. To address this concern we will present data on peptide retention and signal intensity in ProteaseMAX-containing samples. These data will show that ProteaseMAX is safe to use in LC-MS and MALDI under the caveat that the surfactant is used in recommended concentration limits and according to recommended sample handling procedures.


View recording

Thank you for your request for the Promega @cademy Recording/Presentation. To download the recording, please fill out and submit the following form. You will receive an email with the URL where you can download the recording and the presentation.


Entries with * are required.
First Name *
Last Name*
Institution *
Department
Street Address *
Bldg/Room
PO Box Address is for:
City *
State/Region
Country * Postal Code
Telephone Fax
E-Mail *
   
If you recently moved, help us stay current - please tell us where you were before!
Previous Institution Moved here
I give Promega or an authorized Promega distributor permission to contact me at the addresses that I provide.
 
     

Prefer a different language?

Your country is set to {0}
Your language is set to {1}

Select the language that will best suit your needs:

Continue

I need additional help »

  • Warning
  • It appears that you have Javascript disabled. Our website requires Javascript to function correctly. Please enable Javascript to experience our website.