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Advances in gene expression technologies have led to the identification of molecular markers associated with stem cell pluripotency and differentiation. Quantitative PCR, in particular, has proven to be a valuable method in nucleic acid detection; by measuring PCR product accumulation in real time, this technique provides greater sensitivity and more accurate quantitation than traditional endpoint PCR. Coupled with reverse transcription, quantitative PCR has become a powerful tool for characterizing gene expression. Multiplex qPCR facilitates simultaneous amplification of multiple targets by incorporating more than one primer pair in a single reaction. This reduces the number of reactions required for the measurement of multiple transcripts and improves the quality of the experimental data because all the transcripts are measured in the same well. The StemEliteâ„¢ Gene Expression System is a novel real-time PCR system for the detection and relative quantitation of RNA expression levels. The system utilizes validated primer sets to monitor stem cell differentiation using multiplexed amplification.


This webinar was presented by Carl A. Strayer, Ph.D., Technical Services Scientist, Promega Corporation, USA

 


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