Bioluminescence is the
pre-eminent detection method for performing genetic reporter assays. The
traditional reporter of choice has been firefly luciferase, but recently the
need for reporters that are smaller and do not require ATP has resulted in
the increased use of other luciferases, such as the copepod luciferases. The
best studied of this class is Renilla luciferase, the bioluminescent enzyme
derived from the sea pansy, Renilla reniformis. This webinar will focus on
the use of Renilla luciferase in flash assays, dual assays with firefly
luciferase (both flash and glow type), assays with non-lytic live cell
substrates, and a new assay featuring a single-addition reagent that
generates a glow-type signal with Renilla luciferase.
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