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Bioluminescence is the pre-eminent detection method for performing genetic reporter assays. The traditional reporter of choice has been firefly luciferase, but recently the need for reporters that are smaller and do not require ATP has resulted in the increased use of other luciferases, such as the copepod luciferases. The best studied of this class is Renilla luciferase, the bioluminescent enzyme derived from the sea pansy, Renilla reniformis. This webinar will focus on the use of Renilla luciferase in flash assays, dual assays with firefly luciferase (both flash and glow type), assays with non-lytic live cell substrates, and a new assay featuring a single-addition reagent that generates a glow-type signal with Renilla luciferase.

 

 


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