The webinar "Multiplexed In
Vitro Viability and Cytotoxicity Methods for
Strengthening Predictive Confidence and
Improving Mechanistic Content" is a
specialist webinar. Specialist webinars address
more specialized topics and are useful for PhD
students and senior scientists.
A central tenet of pharmacology
is that all compounds have the capacity to induce toxicity due to dosage.
These specific toxicity thresholds are inherently linked to cellular
exposure period and cell-type susceptibility. Therefore, the practical
implementation of any in vitro testing methodology is greatly dependent upon
whether the study is designed to identify targeted cytotoxicity (oncology)
or potential off-target toxicity (safety). Either way, the traditional
experimental paradigm has been to triage data from large primary screening
sets, and then examine the �actives� with a battery of secondary assays in
parallel. This talk will describe numerous same-well multiplex assay
combinations which may obviate parallel assays because they provide
immediate orthogonal or complementary measures useful for confirming
cytotoxicity or flagging non-conforming points for additional study. For
instance, viability and cytotoxicity assays can be added in meaningful
combinations with genetic reporter or cytochrome p450 assays, early measures
of cellular stress and apoptosis, or assays for specific obligate functional
activities. This proposed multiplex paradigm is designed to streamline data
acquisition efficiencies by reducing experimental effort, variability and
adding mechanistic content.
Thank you for your request for the Promega @cademy
Recording/Presentation. To download the recording, please fill out and submit the following form. You will receive an email with the URL where you can download the
recording and the presentation.