"Robust Purification of Arginine Methyltransferase
(CARM1) from Mammalian Cells Using HaloTag Technology"
Identification and characterization of post-translational modifications and
protein function largely depends on success in purification of full-length
target protein. CARM1 is a mammalian transcription factor that plays a role
in progression of breast cancer. CARM1 is able to methylate itself; however,
this process has been previously uncharacterized, due to difficulty in
purifying the full-length protein. HaloTag technology enabled the
purification of full-length, active CARM1 from mammalian cells; the purified
protein was used for both mass spectrometry and functional analysis to
characterize the site of automethylation.
work represents the first identified physiological role for automethylation
in the protein arginine methyltransferase family
Robert S. Chumanov PhD
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