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"Robust Purification of Arginine Methyltransferase (CARM1) from Mammalian Cells Using HaloTag Technology"

Identification and characterization of post-translational modifications and protein function largely depends on success in purification of full-length target protein. CARM1 is a mammalian transcription factor that plays a role in progression of breast cancer. CARM1 is able to methylate itself; however, this process has been previously uncharacterized, due to difficulty in purifying the full-length protein. HaloTag technology enabled the purification of full-length, active CARM1 from mammalian cells; the purified protein was used for both mass spectrometry and functional analysis to characterize the site of automethylation.  This work represents the first identified physiological role for automethylation in the protein arginine methyltransferase family

This webinar is presented by Robert S. Chumanov PhD

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