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Information
"Highly
Efficient
Purification
of
Functional
Proteins
from
Mammalian
Cells
Using
HaloTag"
Although
mammalian
cells
are
preferred
for
producing
functional
mammalian
proteins
with
appropriate
post-translational
modifications,
purification
of
recombinant
proteins
is
typically
hampered
by
low
expression
levels.
We
have
addressed
this
by
creating
a
new
method
configured
for
mammalian
cell
culture,
providing
both
rapid
detection
and
efficient
purification
of
mammalian
proteins
from
their
native
environment.
This
approach
is
based
on a
protein
fusion
tag,
HaloTag,
which
provides
efficient
protein
purification
through
covalent
immobilization
coupled
with
proteolytic
tag
removal.
HaloTag
capabilities
would
be
demonstrated
through
case
studies
of
purifying
multiple
functional
kinases
from
mammalian
cells.
This
webinar
is
presented
by
Rachel
Friedman
Ohana,
Senior
R&D
scientist
at
Promega
Corporation.
View
recording
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