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Genetic reporters are very valuable tools to research. The use of bioluminescence (emission of light that follows the absorption of energy generated by an enzymatically-catalyzed biochemical reaction) as a read-out for genetic reporters (luciferase reporter genes) raises great advantages in terms of sensitivity, background, dynamic range and experimental conditions. The aim of this webinar is first to explain the principle of luciferase reporter assays and how they can be used to generate more reliable data (normalization), and then to review different applications of the luciferase genetic reporter technology using experimental examples. Finally, this webinar addresses step-by-step the important considerations to set up a luciferase genetic reporter assay.
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