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The webinar "Reverse Transcription from Z to A" is a basic webinar. Basic webinars address the basics of specific topics and technologies. They are especially useful for students, technicians and junior scientists in general.



Reverse transcription, generating cDNA from RNA, is a crucial step in many experiments, from cloning to gene expression studies like qPCR. This basic webinar will cover the essentials of reverse transcription, as well as some of the finer points. We will discuss issues related to RNA sample preparation, RT enzyme & reaction considerations, primer choice, and integration of the RT process with downstream assays. We will also address common questions, and common pitfalls.

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