The webinar "Analysis and
molecular detection of microsatellite
instability in MSI-H type cancer cells"
is a specialist webinar. Specialist webinars address
more specialized topics and are useful for PhD
students and senior scientists.
of microsatellites instability is abnormal variation of repetitive
nucleotide sequences in DNA from the tumor compared to DNA of healthy tissue
from the same individual.
whether a tumor exhibits microsatellite instability (MSI) is useful in
identifying patients with hereditary non-polyposis colorectal cancer (HNPCC)
and sporadic gastrointestinal cancers with defective mismatch repair system
(MMR). There is evidence to suggest that colorectal cancer with MSI-H
respond differently to adjuvant chemotherapy so the assessment of MSI-H
status aids in establishing a clinical prognosis.
developed, based on previous studies done by Dr Hamelin and his group, the
MSI analysis system. This fluorescent multiplex PCR system meets the 2002
NCI workshop recommendations and includes five mononucleotides markers
(BAT-25, BAT-26, NR-21, NR-24 and MONO-27). In addition two pentanucleotide
markers were added to identify sample mixs-up and/or contamination. PCR
products are separated by capillary electrophoresis. A MSI analysis macro
software simplifies and standardizes allele size determination for
consistent data analysis.
procedure from DNA extraction to MSI analysis will be described during the
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