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HaloTag® Technology is a platform technology that enables the understanding of protein
function in vivo and in vitro. The technology is based on the efficient formation of a covalent bond between a specially designed HaloTag reporter protein, a 34.1kDa modified hydrolase, and unique HaloTag ligands in living cells, in solution or on a solid support. Coupling this technology with Cell-Free Translation systems allow the researchers to detect, capture, and perform functional assays on solid supports with their protein of interest. In this presentation, we focus on applications of a cell-free expressed kinase, PKA.


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