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Promega Corporation

PowerPlex® Fusion Systems

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PowerPlex® Fusion Systems

Designed to meet CODIS and European standards, the PowerPlex® Fusion Systems enable laboratories to:

  • Achieve the most inter-database compatibility and highest discrimination of any autosomal STR kit.
  • Improve laboratory efficiencies with rapid-cycling and direct-amplification protocols.
  • Obtain a higher success rate with difficult casework samples due to robustness and sensitivity and the inclusion of three Y-STR loci for improved mixture deconvolution results.
  • Simplify validation and QC efforts by using one kit for both casework and databasing sections.

The PowerPlex® Fusion Systems offer the highest discrimination from a single reaction and deliver more information in demanding forensic, paternity and relationship-testing cases.

As DNA databases continue to grow and international cooperation increases, the need for a common set of markers is required to facilitate data sharing and to reduce adventitious matches. The PowerPlex® Fusion and Fusion 6C Systems enable increased discriminatory power and data sharing possibilities by means of the incorporation of common and informative loci used throughout the world, such as SE33 and Penta D and Penta E.

Figure 10946LC

A probability of identity value (PI) is defined as the probability that two individuals selected at random will have an identical genotype at the tested locus. It is calculated by adding the square of the genotype frequencies. The figure indicates the Probability of Identity values for a variety of currently available STR systems and standards. A significant increase in discriminatory power is seen with the PowerPlex® Fusion Systems over other well established STR systems. Data were kindly provided by John Butler (NIST) using data collected by Carolyn Hill and analysis developed by Dave Duewer.

PowerPlex® Fusion System

Figure 10910MA 

Configuration of the PowerPlex® Fusion System. The PowerPlex® Fusion System allows co-amplification and four-color detection of 24 loci, including all CODIS and ESS loci: D3S1358, D1S1656, D2S441, D10S1248, D13S317, D16S539, D18S51, D2S1338, CSF1PO, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, FGA, D22S1045, plus Penta E, Penta D, DYS391 and Amelogenin.

Maximum Sensitivity

The PowerPlex® Fusion System reliability produces complete STR profiles from as little as 100pg of human DNA.

Figure 10911MA

 

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Figure 11018MA

 

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Sensitivity of the PowerPlex® Fusion System. Left panel. Amplification of 100pg of human DNA using 30 cycles and the PowerPlex® Fusion System. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5‑second injection). Right panel. The PowerPlex® Fusion System demonstrates superior sensitivity, generating full profiles with as little as 100pg input DNA.

Superior Inhibitor Tolerance

With a robust buffer system designed to tolerate the most challenging inhibitors, the PowerPlex® Fusion System minimizes the need to re-amplify samples generally thought to be too challenging to run, saving your lab countless hours of repeat analysis.

Figure 10912MB
Inhibitor tolerance. Human genomic DNA (500pg) was amplified with each listed kit in the presence of common PCR inhibitors. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5‑second injection). Percentage of alleles detected is indicated for each system.

Direct-Amplification Compatibility for Most Sample Types

Building on the proven flexibility of our PowerPlex® 21 System, the PowerPlex® Fusion System allows direct amplification from FTA® card punches as well as pretreated nonFTA cards and commonly used swabs to streamline workflow and expand sample throughput.

Figure 10913MA
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Figure 10914MA
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Figure 10915MA
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Figure 10916MB
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Direct amplification using the PowerPlex® Fusion System. Top left panel. One 1.2mm buccal punch of an S&S 903 card was pretreated with PunchSolution™ Reagent as recommended in the PunchSolutionKit Technical Manual TMD038. Top right panel. A swab was pretreated with SwabSolution™ Reagent. Following incubation, 2µl of extract was added to the PCR amplification mix and amplified as described in the PowerPlex® Fusion System Technical Manual TMD039. Bottom left panel. Direct amplification of one 1.2mm FTA® card punch containing blood using the protocol described in the PowerPlex® Fusion System Technical Manual TMD039. Bottom right panel. Direct amplification of two 1.2mm FTA® card punches from a buccal sample using the protocol described in the PowerPlex® Fusion System Technical Manual TMD039. Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5‑second injection).

PowerPlex® Fusion 6C System

Figure 12469MA

Configuration of the PowerPlex® Fusion 6C System. The PowerPlex® Fusion 6C System allows co-amplification and fluorescent detection of 27 loci, including all CODIS and ESS loci: D3S1358, D1S1656, D2S441, D10S1248, D13S317, D16S539, D18S51, D2S1338, CSF1PO, TH01, vWA, D21S11, D7S820, D5S818, TPOX, D8S1179, D12S391, D19S433, SE33, D22S1045, FGA, plus DYS391, DYS576, DYS570, Penta E, Penta D and Amelogenin.

Superior Sensitivity

The PowerPlex® Fusion 6C System reliability produces complete STR profiles from as little as 125pg of human DNA and usable, partial profiles from DNA amounts lower than 125pg.

 

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Sensitivity of the PowerPlex® Fusion 6C System. The average peak heights (vertical bars) and percentage of alleles called (diamonds) at varying amounts of 2800M Control DNA amplified with the PowerPlex® Fusion 6C System (Panel A) or the GlobalFiler® PCR Amplification Kit (Panel B). Error bars show the standard deviation of the mean from triplicate amplification reactions. Amplification reactions were performed using a GeneAmp® PCR System 9700 thermal cycler, and 1μl of amplified products was separated on an Applied Biosystems® 3500 Genetic Analyzer (1.2kV, 15-second injection).

Enhanced Inhibitor Tolerance

The PowerPlex® Fusion 6C System can successfully meet the challenges of inhibitor-laden samples, increasing your success rate and minimizing the need to re-amplify samples.

Figure 12560MA
Inhibitor tolerance. Human genomic DNA (500pg) was amplified with the indicated kits in the presence of common PCR inhibitors. Amplified products were separated on an Applied Biosystem® 3500 Genetic Analyzer (1.2kV, 15-second injection). Percentage of alleles detected is indicated for each system.

Half-Volume Reaction Direct-Amplification Capability

The PowerPlex® Fusion 6C System enables direct amplification with half-volume reactions from numerous sample types, including FTA® card punches, pretreated nonFTA cards (data not shown) and commonly used swabs. Laboratories can streamline their workflow while stretching their budget.

Figure 12562MA
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Figure 12563MA
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Left panel. Direct amplification of two 1.2mm punches from a buccal sample on an FTA® card using the recommended protocol for half-volume reactions. Amplified products were separated using an Applied Biosystems® 3500 Genetic Analyzer (1.2kV, 15-second injection). Right panel. A buccal swab was pretreated with SwabSolution™ Reagent as recommended in the SwabSolutionKit Technical Manual. Following incubation, 2μl of extract was added to the PCR amplification mix using the recommended protocol for half-volume reactions. Amplified products were separated on an Applied Biosystems® 3500 Genetic Analyzer (1.2kV, 15-second injection).

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