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| 26. |
McDaid, H.M., Mani, S., Shen, H-J., Muggia, F., Sonnichsen, D., Horwitz, S.B.
(2002)
Validation of the pharmacodynamics of BMS-247550, an analogue of epothilone B, during a phase I clinical study
Clin. Cancer Res.
8
,
2035-2043
.
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| |
Notes:
The authors investigated the mechansim of action of BMS-247550, a stable, synthetic derivative of epothilone B, in peripheral blood mononuclear cells and in breast tumor cells. Breast tumor cells expressing multidrug resistance were stained for tubulin bundle formation. Cytotoxicity in these biopsied cells also was examined by staining with Promega's Anti-PARP p85 Fragment, pAb.
(0002497) |
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Products: Anti-PARP p85 Fragment pAb | TMB One Solution |
| 27. |
Ackerley, S., Grierson, A.J., Brownlees, J., Thornhill, P., Anderton, B.H., Leigh, P.N., Shaw, C.E., and Miller C.C.
(2000)
Glutamate slows axonal transport of neurofilaments in transfected neurons.
J. Cell Biol.
150
,
165-175
.
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| |
Notes:
The authors seek to determine the role of glutamate in excitotoxicity and neurofilament accumulation seen in some neurodegenerative diseases. Neurofilament light, middle, and heavy chains were expressed from rat cDNAs cloned into the pCI-neo Mammalian Expression vector in SW-13 cells. Primary rat cortical neurons were transfected with a neurofilament middle chain and green fluorescent fusion protein. SW13 cells and primary rat cortical neurons were transfected with the ProFection® Mammalian Transfection System–Calcium Phosphate. The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to monitor glutamate toxicity in these cell. To determine the role of the MAPK and JNK signaling pathways, SW13- cells and primary neuronal cells were immunostained for dually phosphorylated MAPK and JNK using Promega's Anti-ACTIVE® MAPK pAb and Anti-ACTIVE® JNK pAb, respectively. Cells were fixed in 4% paraformaldehyde, permeabilized in 0.1% Triton® X-100 in PBS, blocked with 0.2% Tween® 20 in TBS, and incubated with primary antibodies diluted in blocking solution. Western blot analyses were performed on the primary cortical neurons to quantitate the level of dually phosphorylated MAPK protein The blots were also probed with a pan MAPK antibody that detects total (active and inactive) MAPK.
(0002382) |
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Products: Anti-ACTIVE® JNK pAb, Rabbit, (pTPpY) | Anti-ACTIVE® MAPK pAb, Rabbit, (pTEpY) | CytoTox 96® Non-Radioactive Cytotoxicity Assay | pCI-neo Mammalian Expression Vector | ProFection® Mammalian Transfection System—Calcium Phosphate |
| 28. |
Aliprantis, A.O., Yang, R.-B., Weiss, D.S., Godowski, P. and Zychlinsky, A.
(2000)
The apoptotic signaling pathway activated by Toll-like receptor-2
EMBO J.
19
,
3325-3336
.
|
| |
Notes:
Apoptosis was studied by TUNEL assay using the Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL System) and PI staining. Amount of apoptosis was quantitated by flow cytometry. Test compounds that activate the TLR2 pathway were tested for cytotoxicity using the CytoTox 96® Non-Radioactive Cytotoxicity Assay. The cells were at 2.4x104 cells/well.
(0002128) |
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Products: CytoTox 96® Non-Radioactive Cytotoxicity Assay | DeadEnd™ Fluorometric TUNEL System |
| 29. |
Kim, H.S., Park, C.H., Cha, S.H., Lee, J.H., Lee, S., Kim, Y., Rah, J.C., Jeong, S.J. and Suh, Y.H.
(2000)
Carboxyl-terminal fragment of Alzheimer's APP destabilizes calcium homeostasis and renders neuronal cells vulnerable to excitotoxicity.
FASEB J.
14
,
1508-17
.
|
| |
Notes:
The CytoTox® 96 Non-Radioactive Cytotoxicity Assay was used to determine the toxicity of two peptides, a C-terminal fragment of APP (CT 105), and Amyloid β protein (Aβ 1-42). Cells were treated with the peptides in the presence or absence of neuroprotective agents cholesterol, MK801, nifedipine, or verapamil. Studies were done in PC12 cells and in SK-N-SH human neuroblastoma cells.
(0002134) |
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Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay | CytoTox 96® Non-Radioactive Cytotoxicity Assay |
| 30. |
Sperl, S., Jacob, U., de Prada, N.A., Sturzebecher, J., Wilhelm, O.G., Bode, W., Magdolen, V., Huber, R., Moroder, L.
(2000)
(4-Aminomethyl)phenylguanidine derivatives as nonpeptidic highly selective inhibitors of human urokinase
Proc. Natl. Acad. Sci. U S A
97
,
5113-5118
.
|
| |
Notes:
The authors synthesized a new class of nonpeptidic, reversible inhibitors of the serine protease urokinase-type plasminogen activator (uPA). One of these inhibitors was tested for its cytotoxicity against several human carcinoma cell lines (OV-MZ-6, MDA-MB-231, and A431) using the CellTiter 96® Non-Radioactive Cell Proliferation Assay.
(0002476) |
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Products: CellTiter 96® Non-Radioactive Cell Proliferation Assay |
| 31. |
Morigiwa, K. , Quan, M. , Murakami, M. , Yamashita, M. , and Fukuda, Y.
(2000)
P2 Purinoceptor expression and functional changes of hypoxia-activated cultured rat retinal microglia.
Neurosci. Lett.
282(3)
,
153-156
.
|
| |
Notes:
The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the survival of the rat retinal microglia to P2 receptor agonists and antagonists.
(0000010) |
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Products: CellTiter 96® AQueous One Solution Cell Proliferation Assay |
| 32. |
Heese, K. , Nagai, Y. , and Sawada, T.
(2000)
Induction of rat L-phosphoserine phosphatase by amyloid-beta (1-42) is inhibited by interleukin-11.
Neurosci. Lett.
288(1)
,
37-40
.
|
| |
Notes:
The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to judge the percent survival of B104 rat neuroblastoma cells in response to beta-amyloid peptides as well as glutamate and serine combinations.
(0000015) |
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Products: CellTiter 96® AQueous One Solution Cell Proliferation Assay |
| 33. |
Shankar, P. P. , Wei, H. , Davee, S. M. , and Funk, J. L.
(2000)
Parathyroid hormone-related protein is expressed by transformed and fetal human astrocytes and inhibits cell proliferation.
Brain Res.
868(2)
,
230-240
.
|
| |
Notes:
The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to show that TNFalpha nor EGF were toxic to cultured primary human astrocytes or U-373 MG transformed human astrocytoma cells.
(0000016) |
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Products: CellTiter 96® AQueous One Solution Cell Proliferation Assay |
| 34. |
Tan, N.S., Ng, M.L., Yau, Y.H., Chong, P.K., Ho, B. and Ding, J.L.
(2000)
Definition of endotoxin binding sites in horseshoe crab Factor C recombinant sushi proteins and neutralization of endotoxin by sushi peptides.
FASEB J.
14
,
1801-1813
.
|
| |
Notes:
The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to assess toxicity of peptides in cell culture. The cells were THP-1 monocytes, and the peptide concentrations used were 1.25 to 320µM.
(0002127) |
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Products: CellTiter 96® AQueous One Solution Cell Proliferation Assay |
| 35. |
Malecki, A., Garrido, R., Mattson, M.P., Hennig, B., Toborek, M.
(2000)
4-Hydroxynonenal induces oxidative stress and death of cultured spinal cord neurons
J. Neurochem.
74
,
2278-2287
.
|
| |
Notes:
The authors treated mouse spinal cord neurons with different doses of 4-hydroxynonenal (HNE) and assessed cytotoxicity using the CellTiter 96® AQueous One Solution Cell Proliferation Assay.
(0002533) |
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Products: CellTiter 96® AQueous One Solution Cell Proliferation Assay |
| 36. |
Jung, S. K. , Mai, A. , Iwamoto, M. , Arizono, N. , Fujimoto, D. , Sakamaki, K. , and Yonehara, S.
(2000)
Purification and cloning of an apoptosis-inducing protein derived from fish infected with Anisakis simplex, a causative nematode of human anisakiasis.
J. Immunol.
165(3)
,
1491-1497
.
|
| |
Notes:
The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to assess the cytotoxicity of the fish apoptosis-inducing protein to human HL-60 myeloblasts.
(0000036) |
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Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay |
| 37. |
Lu, Y. , Friedman, R. , Kushner, N. , Doling, A. , Thomas, L. , Touzjian, N. , Starnbach, M. /and Lieberman, J.
(2000)
Genetically modified anthrax lethal toxin safely delivers whole HIV protein antigens into the cytosol to induce T cell immunity.
Proc. Natl. Acad. Sci. U S A
97(14)
,
8027-8032
.
|
| |
Notes:
RAW 264.7 macrophages were challenged with wildtype and recombinant anthrax lethal factor in the presence of protective antigen. Cytotoxicity was compared to untreated cells. Viable cells were measured with the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS).
(0000039) |
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Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay |
| 38. |
Wang, J. L. , Liu, D. , Zhang, Z. J. , Shan, S. , Han, X. , Srinivasula, S. M. , Croce, C. M. , Alnemri, E. S. , and Huang, Z.
(2000)
Structure-based discovery of an organic compound that binds Bcl-2 protein and induces apoptosis of tumor cells.
Proc. Natl. Acad. Sci. U S A
97(13)
,
7124-7129
.
|
| |
Notes:
The response of HL-60 cells to a Bcl-2 binding molecule, HA14-1, was examined with the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS). The compound strongly induced cytotoxicity via apoptosis.
(0000040) |
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Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay |
| 39. |
Stokes, A. H., Lewis, D. Y., Lash, L. H., Jerome, W. G. III, Grant, K. W., Aschner, M., Vrana, K. E.
(2000)
Dopamine toxicity in neuroblastoma cells: role of glutathione depletion by L-BSO and apoptosis.
Brain Res.
858
,
1-8
.
|
| |
Notes:
The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to examine dopamine-induced toxicity. SK-N-SH human neuroblastomas were cultured for 40 hours with and without a glutathione-depleting compound prior to addition of dopamine for 24hours. Other compounds like ascorbate, pyruvate and manganese were tested in combination with the dopamine treatment.
(0000336) |
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Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay |
| 40. |
Nakagawa, T., Zhu, H., Morishima, N., Li, E., Xu, J., Yankner, B.A., Yuan, J.
(2000)
Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta.
Nature
403
,
98-103
.
|
| |
Notes:
Primary mouse cortical neurons derived from caspase-12 normal and knockout mice were tested for cytotoxicity to amyloid beta peptides, staurosporine or a media component. Cytotoxicity was analyzed with the CellTiter 96® AQueous Assay (MTS/PMS).
(0000642) |
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Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay |
| 41. |
Arsura, M., Mercurio, F., Oliver, A.L., Thorgeirsson, S.S. and Sonenshein, G.E.
(2000)
Role of the IkappaB kinase complex in oncogenic ras- and raf-mediated transformation of rat liver epithelial cells.
Mol. Cell. Biol.
20
,
5381-5391
.
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| |
Notes:
The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used in Rat Liver Epithelial cells (RLEs). Response to treatment with TGF-β1 was compared between normal RLE cell lines and RLEs transformed with Ha-ras (F22-ras cell line), or with v-Raf (F3611-T2 and F3611-TH cell lines). TGF-β1 treatment dramatically inhibited growth of the wild type RLEs, but two of the three transformed lines showed only a modest decrease in proliferation. Cells were plated in 96 well dishes at 2x105 cells per well in 100 µl total volume.
(0002133) |
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Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay |
| 42. |
Romach, E.H., Zhao, C.Q., Del Razo, L.M., Cebrian, M.E., Waalkes, M.P.
(2000)
Studies on the mechanisms of arsenic-induced self tolerance developed in liver epithelial cells through continuous low-level arsenite exposure
Toxicol. Sci.
54
,
500-508
.
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| |
Notes:
A culture of TRL 1215 cells was grown for 18-20 weeks in the presence of low levels (500nM) of arsenic to generate a chronic exposed cell line. These cells were tested versus the original cell line for cytotoxicity due to arsenic. The chronically exposed cells were at least a log less sensitive to the chemical. the cytotoxicity was determined with the CellTiter 96® AQueous Cell Proliferation Assay.
(0002546) |
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Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay |
| 43. |
Singer, C.A., Figueroa-Masot, X.A., Batchelor, R.H., Dorsa, D.M.
(1999)
The mitogen-activated protein kinase pathway mediates extrogen neuroprotection after glutamate toxicity in primary cortical neurons.
J. Neurosci.
19
,
2455-2463
.
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| |
Notes:
The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess LDH release from primary cortical neurons following 24hr exposure to glutamate. Various compounds including 2.5S Nerve Growth Factor were assessed for their ability to block the glutamate-induced cytotoxicity. One of the neuroprotective factors, estrogen, was also examined for induction protein tyrosine kinase activity with the the SignaTECT® Protein Tyrosine Kinase Assay System. The induction of PTK activity peaked at 1min and returned to normal within 10min.
(0000367) |
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Products: CytoTox 96® Non-Radioactive Cytotoxicity Assay | mNGF, 2.5S | SignaTECT® Protein Tyrosine Kinase (PTK) Assay System |
| 44. |
Aliprantis, A.O., Yang, R.-B., Mark, M.R., Suggett, S., Devaux, B., Radolf, J.D., Klimpel, G.R., Godowski, P. and Zychlinsky, A.
(1999)
Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2.
Science
285
,
736-739
.
|
| |
Notes:
293 cells stably expressing the human toll-like receptor-2 were challenged with various agents and analyzed for apoptosis. One test was a visual inspection for blebbing and the other was quantitation by TUNEL staining, using the Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL Assay), followed by FACS analysis. In another assay for THP-1 cell death, the CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to monitor LDH release in response to bacterial lipoproteins.
(0001508) |
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Products: CytoTox 96® Non-Radioactive Cytotoxicity Assay | DeadEnd™ Fluorometric TUNEL System |
| 45. |
Lee, D. H. , Macintyre, J. P. , Taylor, G. R. , Wang, E. , Plante, R. K. , Tam, S. S. , Pope, B. L. , Lau, C. Y.
(1999)
Tepoxalin enhances the activity of an antioxidant, pyrrolidine dithiocarbamate, in attenuating tumor necrosis factor alpha-induced apoptosis in WEHI 164 cells.
J. Pharmacol. Exp. Ther.
289
,
1465-1471
.
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Notes:
The CellTiter 96® Assay was used to determine the reduction in TNFα-induced cytotoxicity of known TNFα inhibitors on WEHI-164 cells.
(0000808) |
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Products: CellTiter 96® Non-Radioactive Cell Proliferation Assay |
| 46. |
Phillips, T.A., Ni, J., Pan, G., Ruben, S.M., Wei, Y-F., Pace, J.L., Hunt, J.S.
(1999)
TRAIL (Apo-2L) and TRAIL Receptors in Human Placentas: Implications for Immune Privilege
J. Immunol.
162
,
6053-6059
.
|
| |
Notes:
The authors investigated the potential of the Trail/Trail-R system to protect the placenta against immune cell attack. Jar, JEG-3, U937, THP-1, and HeLa cells were incubated with rTrail for 20 hours, and cytotoxicity was determined using the CellTiter® 96 Non-Radioactive Cell Proliferation Assay.
(0002503) |
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Products: CellTiter 96® Non-Radioactive Cell Proliferation Assay |
| 47. |
Strayer, D. S., Hoek, J. B., Thomas, A. P., White, M. K.
(1999)
Cellular activation by Ca2+ release from stores in the endoplasmic reticulum but not by increased free Ca2+ in the cytosol.
Biochem. J.
344
,
39-46
.
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| |
Notes:
The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to assess the viability of the primary rat type II aveolar pneumocytes to calcium chelator BAPTA. No significant decrease in viability was observed.
(0000345) |
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Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay |
| 48. |
Cehn, Y-N.P., Sharma, S.K., Ramsey, T.M., Jiang, L., Martin, M.S., Baker, K., Adams, P.D., Bair, K.W., Kaelin, W.G.
(1999)
Selective killing of transformed cells by cyclin/cyclin-dependent kinase 2 antagonists
Proc. Natl. Acad. Sci. U S A
96
,
4325-4329
.
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| |
Notes:
Cells were incubated with peptides containing a motif that serves as a docking site for cyclin/cyclin-dependent kinase 2 complexes. The authors measured the viability of cells incubated with these peptides using the CellTiter® 96 AQueous Non-Radioactive Cytotoxicity Assay.
(0002500) |
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Products: CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay |
| 49. |
Bohm, W., Thoma, S., Leithauser, F., Moller, P., Schirmbeck, R. and Reimann, J.
(1998)
T cell-mediated, IFN-gamma-facilitated rejection of murine B16 melanomas.
J. Immunol.
161
,
897-908
.
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| |
Notes:
The authors cloned the Hepatitis B surface antigen coding region into the pCI Mammalian Expression Vector for expression in COS-7 cells. They also used Promega's Terminal Deoxynucleotidyl Transferase (TdT) in a home brew TUNEL assay.
(0001421) |
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Products: Terminal Deoxynucleotidyl Transferase, Recombinant |
| 50. |
Hess, J., Miko, D., Catic, A., Lehmensiek, V., Russell, D.G., Kaufmann, S.H.E.
(1998)
Mycobacterium bovis bacille Calmette-Guérin strains secreting listeriolysin of Listeria monocytogenes.
Proc. Natl. Acad. Sci. U S A
95
,
5299-5304
.
|
| |
Notes:
The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to determine cytotoxicity of J774A.1 macrophages infected with various M. bovis bacille Calmette-Guérin constructs or L. monocytogenes.
(0001042) |
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Products: CytoTox 96® Non-Radioactive Cytotoxicity Assay |