pGeneClip(TM) Neomycin Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGeneClip(TM) Neomycin Vector sequence reference points: Base pairs 4758 T7 RNA polymerase transcription initiation site 1 U1 promoter (human, -392 to +1) 46-438 10bp spacer 439-448 U1 termination sequence 449-465 SP6 RNA polymerase promoter (-17 to +3) 527-546 SP6 RNA polymerase promoter primer binding site 529-547 Binding region of pUC/M13 reverse sequencing primer 564-585 SV40 early enhancer/promoter 798-1216 SV40 minimum origin of replication 1114-1179 Coding region of neomycin phosphotransferase 1251-2045 Synthetic poly(A) signal 2080-2128 Beta-lactamase (Ampr) coding region 3080-3940 Binding region of pUC/M13 forward sequencing primer 4692-4715 T7 RNA polymerase promoter (-17 to +3) 4742-3 .. 1 GGGCGAATTG GGCCCGATAT CTCTAGAGTC GACGAATTCG GATCCCTAAG 51 GACCAGCTTC TTTGGGAGAG AACAGACGCA GGGGCGGGAG GGAAAAAGGG 101 AGAGGCAGAC GTCACTTCCC CTTGGCGGCT CTGGCAGCAG ATTGGTCGGT 151 TGAGTGGCAG AAAGGCAGAC GGGGACTGGG CAAGGCACTG TCGGTGACAT 201 CACGGACAGG GCGACTTCTA TGTAGATGAG GCAGCGCAGA GGCTGCTGCT 251 TCGCCACTTG CTGCTTCACC ACGAAGGAGT TCCCGTGCCC TGGGAGCGGG 301 TTCAGGACCG CTGATCGGAA GTGAGAATCC CAGCTGTGTG TCAGGGCTGG 351 AAAGGGCTCG GGAGTGCGCG GGGCAAGTGA CCGTGTGTGT AAAGAGTGAG 401 GCGTATGAGG CTGTGTCGGG GCAGAGGCCC AAGATCTCGC AGTCTGGAGT 451 TTCAAAAGTA GACTGGGCGG CCGCATCGAT GTTAACCTCG AGGAGCTCCC 501 AACGCGTTGG ATGCATAGCT TGAGTATTCT ATAGTGTCAC CTAAATAGCT 551 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 601 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 651 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 701 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 751 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTGATCTGCG 801 CAGCACCATG GCCTGAAATA ACCTCTGAAA GAGGAACTTG GTTAGGTACC 851 TTCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG 901 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 951 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 1001 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT 1051 AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC 1101 CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 1151 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG 1201 CTTTTGCAAA AAGCTTGATT CTTCTGACGC TAGCGATCGC CCGGGCCACC 1251 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 1301 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG 1351 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 1401 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT 1451 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG 1501 TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 1551 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 1601 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG 1651 ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC 1701 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC 1751 AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC 1801 TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 1851 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 1901 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG 1951 GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 2001 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGACTCGA 2051 GTTTAAACTC TAGAACCGGT CATGGCCGCA ATAAAATATC TTTATTTTCA 2101 TTACATCTGT GTGTTGGTTT TTTGTGTGTT CGAACTAGAA GCTTGCTTCC 2151 TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC 2201 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG 2251 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA 2301 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 2351 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 2401 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT 2451 CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 2501 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 2551 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC 2601 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 2651 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 2701 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC 2751 TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 2801 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 2851 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA 2901 GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 2951 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 3001 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA 3051 AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA 3101 GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 3151 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC 3201 AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC 3251 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 3301 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA 3351 AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG 3401 CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 3451 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG 3501 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT 3551 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 3601 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC 3651 TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG 3701 GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 3751 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC 3801 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC 3851 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 3901 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT 3951 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 4001 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 4051 TTCCCCGAAA AGTGCCACCT GATGCGGTGT GAAATACCGC ACAGATGCGT 4101 AAGGAGAAAA TACCGCATCA GGAAATTGTA AGCGTTAATA TTTTGTTAAA 4151 ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG 4201 AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG 4251 AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC 4301 CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG 4351 AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA 4401 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC 4451 GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA 4501 GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC 4551 GCGCTTAATG CGCCGCTACA GGGCGCGTCC ATTCGCCATT CAGGCTGCGC 4601 AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT 4651 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT 4701 TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGAATT GTAATACGAC 4751 TCACTATA Sequence file prepared 5-Oct-04.