pGeneClip(TM) Hygromycin Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGeneClip(TM) Hygromycin Vector sequence reference points: Base pairs 4989 T7 RNA polymerase transcription initiation site 1 U1 promoter (human, -392 to +1) 46-438 10bp spacer 439-448 U1 termination sequence 449-465 SP6 RNA polymerase promoter (-17 to +3) 527-546 SP6 RNA polymerase promoter binding site 529-547 Binding region of pUC/M13 reverse sequencing primer 564-585 SV40 early enhancer/promoter 798-1216 SV40 minimum origin of replication 1114-1179 Hygromycin phosphotransferase coding region 1251-2276 Synthetic poly(A) signal 2311-2359 Beta-lactamase (Ampr) coding region 3311-4171 Reporter Vector primer 3 (RVprimer3) binding region 4191-4210 .. 1 GGGCGAATTG GGCCCGATAT CTCTAGAGTC GACGAATTCG GATCCCTAAG 51 GACCAGCTTC TTTGGGAGAG AACAGACGCA GGGGCGGGAG GGAAAAAGGG 101 AGAGGCAGAC GTCACTTCCC CTTGGCGGCT CTGGCAGCAG ATTGGTCGGT 151 TGAGTGGCAG AAAGGCAGAC GGGGACTGGG CAAGGCACTG TCGGTGACAT 201 CACGGACAGG GCGACTTCTA TGTAGATGAG GCAGCGCAGA GGCTGCTGCT 251 TCGCCACTTG CTGCTTCACC ACGAAGGAGT TCCCGTGCCC TGGGAGCGGG 301 TTCAGGACCG CTGATCGGAA GTGAGAATCC CAGCTGTGTG TCAGGGCTGG 351 AAAGGGCTCG GGAGTGCGCG GGGCAAGTGA CCGTGTGTGT AAAGAGTGAG 401 GCGTATGAGG CTGTGTCGGG GCAGAGGCCC AAGATCTCGC AGTCTGGAGT 451 TTCAAAAGTA GACTGGGCGG CCGCATCGAT GTTAACCTCG AGGAGCTCCC 501 AACGCGTTGG ATGCATAGCT TGAGTATTCT ATAGTGTCAC CTAAATAGCT 551 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 601 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 651 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 701 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 751 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTGATCTGCG 801 CAGCACCATG GCCTGAAATA ACCTCTGAAA GAGGAACTTG GTTAGGTACC 851 TTCTGAGGCG GAAAGAACCA GCTGTGGAAT GTGTGTCAGT TAGGGTGTGG 901 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA 951 ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 1001 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT 1051 AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC 1101 CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCG 1151 GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG 1201 CTTTTGCAAA AAGCTTGATT CTTCTGACGC TAGCGATCGC CCGGGCCACC 1251 ATGAAAAAGC CTGAACTCAC CGCGACGTCT GTCGAGAAGT TTCTGATCGA 1301 AAAGTTCGAC AGCGTGTCCG ACCTGATGCA GCTCTCGGAG GGCGAAGAAT 1351 CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA 1401 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT 1451 TGCATCGGCC GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA 1501 GCGAGAGCCT GACCTATTGC ATCTCCCGCC GTGCACAGGG TGTCACGTTG 1551 CAAGACCTGC CTGAAACCGA ACTGCCCGCT GTTCTGCAGC CGGTCGCGGA 1601 GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG AGCGGGTTCG 1651 GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC 1701 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA 1751 CGACACCGTC AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT 1801 GGGCCGAGGA CTGCCCCGAA GTCCGGCACC TCGTGCACGC GGATTTCGGC 1851 TCCAACAATG TCCTGACGGA CAATGGCCGC ATAACAGCGG TCATTGACTG 1901 GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC AACATCTTCT 1951 TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG CTACTTCGAG 2001 CGGAGGCATC CGGAGCTTGC AGGATCGCCG CGGCTCCGGG CGTATATGCT 2051 CCGCATTGGT CTTGACCAAC TCTATCAGAG CTTGGTTGAC GGCAATTTCG 2101 ATGATGCAGC TTGGGCGCAG GGTCGATGCG ACGCAATCGT CCGATCCGGA 2151 GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG CGGCCGTCTG 2201 GACCGATGGC TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA 2251 GCACTCGTCC GAGGGCAAAG GAATAGCTCG AGTTTAAACT CTAGAACCGG 2301 TCATGGCCGC AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT 2351 TTTTGTGTGT TCGAACTAGA AGCTTGCTTC CTCGCTCACT GACTCGCTGC 2401 GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA 2451 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 2501 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 2551 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 2601 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 2651 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 2701 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC 2751 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 2801 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 2851 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 2901 GCAGCCACTG GTAACGGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 2951 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 3001 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 3051 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 3101 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 3151 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 3201 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 3251 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 3301 GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC 3351 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 3401 TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 3451 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC 3501 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 3551 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 3601 GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG 3651 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 3701 TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 3751 CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG 3801 GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 3851 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 3901 GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT 3951 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 4001 ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 4051 GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG 4101 TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 4151 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 4201 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 4251 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 4301 TGATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA ATACCGCATC 4351 AGGAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT 4401 TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA 4451 TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA 4501 ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA 4551 ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCATCAC CCTAATCAAG 4601 TTTTTTGGGG TCGAGGTGCC GTAAAGCACT AAATCGGAAC CCTAAAGGGA 4651 GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT GGCGAGAAAG 4701 GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC 4751 GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC 4801 AGGGCGCGTC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT 4851 CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT 4901 GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG 4951 TAAAACGACG GCCAGTGAAT TGTAATACGA CTCACTATA Sequence file prepared 05-Oct-04.