pGeneClip(TM) hMGFP Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGeneClip(TM) hMGFP Vector sequence reference points: Base pairs 5267 T7 RNA polymerase transcription initiation site 1 U1 promoter (human, -392 to +1) 46-438 10bp spacer 439-448 U1 termination sequence 449-465 SP6 RNA polymerase promoter (-17 to +3) 527-546 SP6 RNA polymerase promoter primer binding site 529-547 Binding region of pUC/M13 reverse sequencing primer 564-585 CMV enhancer/promoter 801-1550 Chimeric intron 1690-1822 hMGFP open reading frame 1880-2563 Synthetic poly(A) signal 2589-2637 Beta-lactamase (Ampr) coding region 3589-4449 Binding region of pUC/M13 forward sequencing primer 5201-5224 T7 RNA polymerase promoter (-17 to +3) 5250-3 .. 1 GGGCGAATTG GGCCCGATAT CTCTAGAGTC GACGAATTCG GATCCCTAAG 51 GACCAGCTTC TTTGGGAGAG AACAGACGCA GGGGCGGGAG GGAAAAAGGG 101 AGAGGCAGAC GTCACTTCCC CTTGGCGGCT CTGGCAGCAG ATTGGTCGGT 151 TGAGTGGCAG AAAGGCAGAC GGGGACTGGG CAAGGCACTG TCGGTGACAT 201 CACGGACAGG GCGACTTCTA TGTAGATGAG GCAGCGCAGA GGCTGCTGCT 251 TCGCCACTTG CTGCTTCACC ACGAAGGAGT TCCCGTGCCC TGGGAGCGGG 301 TTCAGGACCG CTGATCGGAA GTGAGAATCC CAGCTGTGTG TCAGGGCTGG 351 AAAGGGCTCG GGAGTGCGCG GGGCAAGTGA CCGTGTGTGT AAAGAGTGAG 401 GCGTATGAGG CTGTGTCGGG GCAGAGGCCC AAGATCTCGC AGTCTGGAGT 451 TTCAAAAGTA GACTGGGCGG CCGCATCGAT GTTAACCTCG AGGAGCTCCC 501 AACGCGTTGG ATGCATAGCT TGAGTATTCT ATAGTGTCAC CTAAATAGCT 551 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 601 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG 651 TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 701 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA 751 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTCTTCCGCT 801 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 851 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 901 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 951 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 1001 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 1051 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 1101 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 1151 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 1201 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 1251 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 1301 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 1351 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 1401 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 1451 GTAACAACTG CGATCGCCCG CCCCGTTGAC GCAAATGGGC GGTAGGCGTG 1501 TACGGTGGGA GGTCTATATA AGCAGAGCTC GTTTAGTGAA CCGTCAGATC 1551 ACTAGAAGCT TTATTGCGGT AGTTTATCAC AGTTAAATTG CTAACGCAGT 1601 CAGTGCTTCT GACACAACAG TCTCGAACTT AAGCTGCAGT GACTCTCTTA 1651 AGGTAGCCTT GCAGAAGTTG GTCGTGAGGC ACTGGGCAGG TAAGTATCAA 1701 GGTTACAAGA CAGGTTTAAG GAGACCAATA GAAACTGGGC TTGTCGAGAC 1751 AGAGAAGACT CTTGCGTTTC TGATAGGCAC CTATTGGTCT TACTGACATC 1801 CACTTTGCCT TTCTCTCCAC AGGTGTCCAC TCCCAGTTCA ATTACAGCTC 1851 GCTAGCGATC GCCCGGGGAT ATCGCCACCA TGGGCGTGAT CAAGCCCGAC 1901 ATGAAGATCA AGCTGCGGAT GGAGGGCGCC GTGAACGGCC ACAAATTCGT 1951 GATCGAGGGC GACGGGAAAG GCAAGCCCTT TGAGGGTAAG CAGACTATGG 2001 ACCTGACCGT GATCGAGGGC GCCCCCCTGC CCTTCGCTTA TGACATTCTC 2051 ACCACCGTGT TCGACTACGG TAACCGTGTC TTCGCCAAGT ACCCCAAGGA 2101 CATCCCTGAC TACTTCAAGC AGACCTTCCC CGAGGGCTAC TCGTGGGAGC 2151 GAAGCATGAC ATACGAGGAC CAGGGAATCT GTATCGCTAC AAACGACATC 2201 ACCATGATGA AGGGTGTGGA CGACTGCTTC GTGTACAAAA TCCGCTTCGA 2251 CGGGGTCAAC TTCCCTGCTA ATGGCCCGGT GATGCAGCGC AAGACCCTAA 2301 AGTGGGAGCC CAGTACCGAG AAGATGTACG TGCGGGACGG CGTACTGAAG 2351 GGCGATGTTA ATATGGCACT GCTCTTGGAG GGAGGCGGCC ACTACCGCTG 2401 CGACTTCAAG ACCACCTACA AAGCCAAGAA GGTGGTGCAG CTTCCCGACT 2451 ACCACTTCGT GGACCACCGC ATCGAGATCG TGAGCCACGA CAAGGACTAC 2501 AACAAAGTCA AGCTGTACGA GCACGCCGAA GCCCACAGCG GACTACCCCG 2551 CCAGGCCGGC TAATAGTTCT AGAACCGGTC ATGGCCGCAA TAAAATATCT 2601 TTATTTTCAT TACATCTGTG TGTTGGTTTT TTGTGTGTTC GAACTAGAAG 2651 CTTGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 2701 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG 2751 GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG 2801 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 2851 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA 2901 CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC 2951 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 3001 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT 3051 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT 3101 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 3151 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACGGGATTA 3201 GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT 3251 AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 3301 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA 3351 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC 3401 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 3451 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT 3501 CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA 3551 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 3601 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG 3651 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA 3701 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC 3751 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG 3801 GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA 3851 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 3901 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA 3951 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC 4001 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 4051 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA 4101 CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC 4151 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 4201 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA 4251 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG 4301 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 4351 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA 4401 ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA 4451 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 4501 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC 4551 CGCGCACATT TCCCCGAAAA GTGCCACCTG ATGCGGTGTG AAATACCGCA 4601 CAGATGCGTA AGGAGAAAAT ACCGCATCAG GAAATTGTAA GCGTTAATAT 4651 TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC 4701 AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG 4751 ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA 4801 CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC 4851 CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT 4901 AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG 4951 GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG 5001 CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC 5051 ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCCA TTCGCCATTC 5101 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT 5151 ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA 5201 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTG 5251 TAATACGACT CACTATA Sequence file prepared 5-Oct-04.