pGloSensor(TM)-10F Linear Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGloSensor(TM)-10F Linear Vector sequence reference points: Base pairs 4968 SP6 RNA polymerase promoter (-17 to +1) 23-42 T7 RNA polymerase promoter (-17 to +1)* 46-63 Barley yellow dwarf virus (BYDV) 5' untranslated region 65-201 SgfI recognition site 202-209 GloSensor(TM) protein-coding region 211-1848 NheI recognition site 1147-1152 BglII recognition site (destroyed during cloning) 1153-1158 PmeI recognition site 1849-1856 BYDV 3' translation enhancer 1875-1981 T7 terminator 2086-2133 Beta-lactamase (Ampr) coding region 2467-3327 origin of replication 3482-3518 cer site (site for E. coli XerCD recombinase) 4189-4474 rrnB transcription terminator 4525-4926 *The consensus T7 RNA polymerase promoter sequence extends only to +1 and does not match many standard T7 promoter primers available for sequencing applications. .. 1 GGATCTCGAT CCCGCGAAAT TAATTTAGGT GACACTATAG AAGGGTAATA 51 CGACTCACTA TAGCAGTGAA GATTGACCAT CTCACAAAAG CTGTTACGTG 101 CTTGTAACAC ACTACACACT CGTTTTGTAT TCGAGAAGTA GTTGCAACAA 151 CGGTCCCCTT ATTGCCTGAC AAGCTGAGGG CCACCCTTCT ATCCCCACCG 201 CGCGATCGCC ATGGACACCG CTATCCTCAG CGTGGTGCCA TTTCACCACG 251 GCTTCGGCAT GTTCACCACG CTGGGCTACT TGATCTGCGG CTTTCGGGTC 301 GTGCTCATGT ACCGCTTCGA GGAGGAGCTA TTCTTGCGCA GCTTGCAAGA 351 CTATAAGATT CAATCTGCCC TGCTGGTGCC CACACTATTT AGCTTCTTCG 401 CTAAGAGCAC TCTCATCGAC AAGTACGACC TAAGCAACTT GCACGAGATC 451 GCCAGCGGCG GGGCGCCGCT CAGCAAGGAG GTAGGTGAGG CCGTGGCCAA 501 ACGCTTCCAC CTACCAGGCA TCCGCCAGGG CTACGGCCTG ACAGAAACAA 551 CCAGCGCCAT TCTGATCACC CCCGAAGGGG ACGACAAGCC TGGCGCAGTA 601 GGCAAGGTGG TGCCCTTCTT CGAGGCTAAG GTGGTGGACT TGGACACTGG 651 TAAGACACTG GGTGTGAACC AGCGCGGCGA GCTGTGCGTC CGTGGCCCCA 701 TGATCATGAG CGGCTACGTT AACAACCCCG AGGCTACAAA CGCTCTCATC 751 GACAAGGACG GCTGGCTGCA CAGCGGCGAC ATCGCCTACT GGGACGAGGA 801 CGAGCACTTC TTCATCGTGG ACCGGCTGAA GAGCCTGATC AAATACAAGG 851 GCTACCAGGT AGCCCCAGCC GAACTGGAGA GCATCCTGCT GCAACACCCC 901 AACATCTTCG ACGCCGGGGT CGCCGGCCTG CCCGACGACG ATGCCGGCGA 951 GCTGCCCGCC GCAGTCGTCG TGCTGGAACA CGGTAAAACC ATGACCGAGA 1001 AGGAGATCGT GGACTATGTG GCCAGCCAGG TTACAACCGC CAAGAAGCTG 1051 CGCGGTGGTG TTGTGTTCGT GGACGAGGTG CCTAAAGGAC TGACCGGCAA 1101 GTTGGACGCC CGCAAGATCC GCGAGATTCT CATTAAGGCC AAGAAGGCTA 1151 GCAGATCTGC CAAAAACATT AAGAAGGGCC CAGCGCCATT CTACCCACTC 1201 GAAGACGGGA CCGCCGGCGA GCAGCTGCAC AAAGCCATGA AGCGCTACGC 1251 CCTGGTGCCC GGCACCATCG CCTTTACCGA CGCACATATC GAGGTGGACA 1301 TTACCTACGC CGAGTACTTC GAGATGAGCG TTCGGCTGGC AGAAGCTATG 1351 AAGCGCTATG GGCTGAATAC AAACCATCGG ATCGTGGTGT GCAGCGAGAA 1401 TAGCTTGCAG TTCTTCATGC CCGTGTTGGG TGCCCTGTTC ATCGGTGTGG 1451 CTGTGGCCCC AGCTAACGAC ATCTACAACG AGCGCGAGCT GCTGAACAGC 1501 ATGGGCATCA GCCAGCCCAC CGTCGTATTC GTGAGCAAGA AAGGGCTGCA 1551 AAAGATCCTC AACGTGCAAA AGAAGCTACC GATCATACAA AAGATCATCA 1601 TCATGGATAG CAAGACCGAC TACCAGGGCT TCCAAAGCAT GTACACCTTC 1651 GTGACTTCCC ATTTGCCACC CGGCTTCAAC GAGTACGACT TCGTGCCCGA 1701 GAGCTTCGAC CGGGACAAAA CCATCGCCCT GATCATGAAC AGTAGTGGCA 1751 GTACCGGATT GCCCAAGGGC GTAGCCCTAC CGCACCGCAC CGCTTGTGTC 1801 CGATTCAGTC ATGCCCGCGA CCCCATCTTC GGCAACCAGA TCATCCCCGT 1851 TTAAACGAAT TCGAGCTCGG TACCAGTGAA GACAACACCA CTAGCACAAA 1901 TCGGATCCTG GGAAACAGGC AGAACTTCGG TTCATAAGCT CGGGTAGGCT 1951 GTCAACCTAC CGCCGTATCG TATTGTGTTT GTCTAGATAC GTATCGCGAG 2001 TCGACCTGCA GGCATGCAAG CTGATCCGGC TGCTAACAAA GCCCGAAAGG 2051 AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT 2101 GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT 2151 ATCCGGTTCG CTTGCTGTCC ATAAAACCGC CCAGTCTAGC TATCGCCATG 2201 TAAGCCCACT GCAAGCTACC TGCTTTCTCT TTGCGCTTGC GTTTTCCCTT 2251 GTCCAGATAG CCCAGTAGCT GACATTCATC CGGGGTCAGC ACCGTTTCTG 2301 CGGACTGGCT TTCTACGTAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC 2351 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA 2401 AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA 2451 TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC 2501 CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG 2551 GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT 2601 CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG 2651 AACGTTTTCC AATGATGAGC ACTTTCAAAG TTCTGCTATG TGGCGCGGTA 2701 TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA 2751 TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA 2801 CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT 2851 GATAACACTG CGGCCAACTT ACTTCTGACA ACTATCGGAG GACCGAAGGA 2901 GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC 2951 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC 3001 ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA 3051 ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG 3101 ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT 3151 ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC 3201 AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA 3251 CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA 3301 GGTGCCTCAC TGATTAAGCA TTGGTAATTC GAAATGACCG ACCAAGCGAC 3351 GCCCAACCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 3401 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 3451 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC 3501 CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 3551 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 3601 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT 3651 CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 3701 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 3751 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC 3801 AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 3851 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 3901 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG 3951 CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 4001 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 4051 CGCGCAGAAA AAAAGGATTT CAAGAAGATC CTTTGATCTT TTCTACGGGG 4101 TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 4151 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTATAGTCC GGAAATACAG 4201 GAACGCACGC TGGATGGCCC TTCGCTGGGA TGGTGAAACC ATGAAAAATG 4251 GCAGCTTCAG TGGATTAAGT GGGGGTAATG TGGCCTGTAC CCTCTGGTTG 4301 CATAGGTATT CATACGGTTA AAATTTATCA GGCGCGATTG CGGCAGTTTT 4351 TCGGGTGGTT TGTTGCCATT TTTACCTGTC TGCTGCCGTG ATCGCGCTGA 4401 ACGCGTTTTA GCGGTGCGTA CAATTAAGGG ATTATGGTAA ATCCACTTAC 4451 TGTCTGCCCT CGTAGCCATC GAGATAAACC GCAGTACTCC GGCCACGATG 4501 CGTCCGGCGT AGAGGATCGG GATCTTTTCA GCCTGATACA GATTAAATCA 4551 GAACGCAGAA GCGGTCTGAT AAAACAGAAT TTGCCTGGCG GCAGTAGCGC 4601 GGTGGTCCCA CCTGACCCCA TGCCGAACTC AGAAGTGAAA CGCCGTAGCG 4651 CCGATGGTAG TGTGGGGTCT CCCCATGCGA GAGTAGGGAA CTGCCAGGCA 4701 TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT CGTTTTATCT 4751 GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC GCCGGGAGCG 4801 GATTTGAACG TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG CAGGACGCCC 4851 GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC CTGACGGATG 4901 GCCTTTTTGC GTTTCTACAA ACTCTTTTGT TTATTTTTCT AAATACATTC 4951 AAATATGTAT CCGCTCAT File updated 7-Nov-08.