pGL4.50[luc2/CMV/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGL4.50[luc2/CMV/Hygro] Vector sequence reference points: Base pairs 6600 CMV immediate early enhancer/promoter 14-755 luc2 luciferase reporter gene 859-2511 SV40 late poly(A) region 2546-2767 SV40 early enhancer/promoter 2815-3233 Synthetic hygromycin (Hygr) coding region 3258-4295 Synthetic poly(A) region 4319-4367 Reporter vector primer 4 (RVprimer4) binding region 4434-4453 Replication origin 4691 Synthetic Beta-lactamase (Ampr) coding region 5482-6342 Synthetic poly(A) signal/transcriptional pause region 6447-6600 Reporter Vector primer 3 (RVprimer3) binding region 6549-6568 .. 1 GGCCTAACTG GCCTCAATAT TGGCCATTAG CCATATTATT CATTGGTTAT 51 ATAGCATAAA TCAATATTGG CTATTGGCCA TTGCATACGT TGTATCTATA 101 TCATAATATG TACATTTATA TTGGCTCATG TCCAATATGA CCGCCATGTT 151 GGCATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA 201 GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG 251 CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA 301 CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG 351 GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA 401 TATGCCAAGT CCGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT 451 GGCATTATGC CCAGTACATG ACCTTACGGG ACTTTCCTAC TTGGCAGTAC 501 ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA 551 CACCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC 601 ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC 651 TTTCCAAAAT GTCGTAATAA CCCCGCCCCG TTGACGCAAA TGGGCGGTAG 701 GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC 751 AGATCACTAG AAGCTTTATT GCGGTAGTTT ATCACAGTTA AATTGCTAAC 801 GCAGTCAGTG GGCCTCGGCG GCCAAGCTTG GCAATCCGGT ACTGTTGGTA 851 AAGCCACCAT GGAAGATGCC AAAAACATTA AGAAGGGCCC AGCGCCATTC 901 TACCCACTCG AAGACGGGAC CGCCGGCGAG CAGCTGCACA AAGCCATGAA 951 GCGCTACGCC CTGGTGCCCG GCACCATCGC CTTTACCGAC GCACATATCG 1001 AGGTGGACAT TACCTACGCC GAGTACTTCG AGATGAGCGT TCGGCTGGCA 1051 GAAGCTATGA AGCGCTATGG GCTGAATACA AACCATCGGA TCGTGGTGTG 1101 CAGCGAGAAT AGCTTGCAGT TCTTCATGCC CGTGTTGGGT GCCCTGTTCA 1151 TCGGTGTGGC TGTGGCCCCA GCTAACGACA TCTACAACGA GCGCGAGCTG 1201 CTGAACAGCA TGGGCATCAG CCAGCCCACC GTCGTATTCG TGAGCAAGAA 1251 AGGGCTGCAA AAGATCCTCA ACGTGCAAAA GAAGCTACCG ATCATACAAA 1301 AGATCATCAT CATGGATAGC AAGACCGACT ACCAGGGCTT CCAAAGCATG 1351 TACACCTTCG TGACTTCCCA TTTGCCACCC GGCTTCAACG AGTACGACTT 1401 CGTGCCCGAG AGCTTCGACC GGGACAAAAC CATCGCCCTG ATCATGAACA 1451 GTAGTGGCAG TACCGGATTG CCCAAGGGCG TAGCCCTACC GCACCGCACC 1501 GCTTGTGTCC GATTCAGTCA TGCCCGCGAC CCCATCTTCG GCAACCAGAT 1551 CATCCCCGAC ACCGCTATCC TCAGCGTGGT GCCATTTCAC CACGGCTTCG 1601 GCATGTTCAC CACGCTGGGC TACTTGATCT GCGGCTTTCG GGTCGTGCTC 1651 ATGTACCGCT TCGAGGAGGA GCTATTCTTG CGCAGCTTGC AAGACTATAA 1701 GATTCAATCT GCCCTGCTGG TGCCCACACT ATTTAGCTTC TTCGCTAAGA 1751 GCACTCTCAT CGACAAGTAC GACCTAAGCA ACTTGCACGA GATCGCCAGC 1801 GGCGGGGCGC CGCTCAGCAA GGAGGTAGGT GAGGCCGTGG CCAAACGCTT 1851 CCACCTACCA GGCATCCGCC AGGGCTACGG CCTGACAGAA ACAACCAGCG 1901 CCATTCTGAT CACCCCCGAA GGGGACGACA AGCCTGGCGC AGTAGGCAAG 1951 GTGGTGCCCT TCTTCGAGGC TAAGGTGGTG GACTTGGACA CCGGTAAGAC 2001 ACTGGGTGTG AACCAGCGCG GCGAGCTGTG CGTCCGTGGC CCCATGATCA 2051 TGAGCGGCTA CGTTAACAAC CCCGAGGCTA CAAACGCTCT CATCGACAAG 2101 GACGGCTGGC TGCACAGCGG CGACATCGCC TACTGGGACG AGGACGAGCA 2151 CTTCTTCATC GTGGACCGGC TGAAGAGCCT GATCAAATAC AAGGGCTACC 2201 AGGTAGCCCC AGCCGAACTG GAGAGCATCC TGCTGCAACA CCCCAACATC 2251 TTCGACGCCG GGGTCGCCGG CCTGCCCGAC GACGATGCCG GCGAGCTGCC 2301 CGCCGCAGTC GTCGTGCTGG AACACGGTAA AACCATGACC GAGAAGGAGA 2351 TCGTGGACTA TGTGGCCAGC CAGGTTACAA CCGCCAAGAA GCTGCGCGGT 2401 GGTGTTGTGT TCGTGGACGA GGTGCCTAAA GGACTGACCG GCAAGTTGGA 2451 CGCCCGCAAG ATCCGCGAGA TTCTCATTAA GGCCAAGAAG GGCGGCAAGA 2501 TCGCCGTGTA ATAATTCTAG AGTCGGGGCG GCCGGCCGCT TCGAGCAGAC 2551 ATGATAAGAT ACATTGATGA GTTTGGACAA ACCACAACTA GAATGCAGTG 2601 AAAAAAATGC TTTATTTGTG AAATTTGTGA TGCTATTGCT TTATTTGTAA 2651 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT 2701 ATGTTTCAGG TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA 2751 CCTCTACAAA TGTGGTAAAA TCGATAAGGA TCCGTTTGCG TATTGGGCGC 2801 TCTTCCGCTG ATCTGCGCAG CACCATGGCC TGAAATAACC TCTGAAAGAG 2851 GAACTTGGTT AGCTACCTTC TGAGGCGGAA AGAACCAGCT GTGGAATGTG 2901 TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 2951 GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG 3001 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 3051 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG 3101 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG 3151 AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC 3201 TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCGATTCTT CTGACACTAG 3251 CGCCACCATG AAGAAGCCCG AACTCACCGC TACCAGCGTT GAAAAATTTC 3301 TCATCGAGAA GTTCGACAGT GTGAGCGACC TGATGCAGTT GTCGGAGGGC 3351 GAAGAGAGCC GAGCCTTCAG CTTCGATGTC GGCGGACGCG GCTATGTACT 3401 GCGGGTGAAT AGCTGCGCTG ATGGCTTCTA CAAAGACCGC TACGTGTACC 3451 GCCACTTCGC CAGCGCTGCA CTACCCATCC CCGAAGTGTT GGACATCGGC 3501 GAGTTCAGCG AGAGCCTGAC ATACTGCATC AGTAGACGCG CCCAAGGCGT 3551 TACTCTCCAA GACCTCCCCG AAACAGAGCT GCCTGCTGTG TTACAGCCTG 3601 TCGCCGAAGC TATGGATGCT ATTGCCGCCG CCGACCTCAG TCAAACCAGC 3651 GGCTTCGGCC CATTCGGGCC CCAAGGCATC GGCCAGTACA CAACCTGGCG 3701 GGATTTCATT TGCGCCATTG CTGATCCCCA TGTCTACCAC TGGCAGACCG 3751 TGATGGACGA CACCGTGTCC GCCAGCGTAG CTCAAGCCCT GGACGAACTG 3801 ATGCTGTGGG CCGAAGACTG TCCCGAGGTG CGCCACCTCG TCCATGCCGA 3851 CTTCGGCAGC AACAACGTCC TGACCGACAA CGGCCGCATC ACCGCCGTAA 3901 TCGACTGGTC CGAAGCTATG TTCGGGGACA GTCAGTACGA GGTGGCCAAC 3951 ATCTTCTTCT GGCGGCCCTG GCTGGCTTGC ATGGAGCAGC AGACTCGCTA 4001 CTTCGAGCGC CGGCATCCCG AGCTGGCCGG CAGCCCTCGT CTGCGAGCCT 4051 ACATGCTGCG CATCGGCCTG GATCAGCTCT ACCAGAGCCT CGTGGACGGC 4101 AACTTCGACG ATGCTGCCTG GGCTCAAGGC CGCTGCGATG CCATCGTCCG 4151 CAGCGGGGCC GGCACCGTCG GTCGCACACA AATCGCTCGC CGGAGCGCAG 4201 CCGTATGGAC CGACGGCTGC GTCGAGGTGC TGGCCGACAG CGGCAACCGC 4251 CGGCCCAGTA CACGACCGCG CGCTAAGGAG GTAGGTCGAG TTTAAACTCT 4301 AGAACCGGTC ATGGCCGCAA TAAAATATCT TTATTTTCAT TACATCTGTG 4351 TGTTGGTTTT TTGTGTGTTC GAACTAGATG CTGTCGACCG ATGCCCTTGA 4401 GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC 4451 GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT 4501 GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC 4551 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 4601 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 4651 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 4701 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 4751 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 4801 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 4851 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 4901 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 4951 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 5001 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 5051 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 5101 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC 5151 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 5201 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 5251 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 5301 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 5351 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 5401 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC 5451 AGCGGCCGCA AATGCTAAAC CACTGCAGTG GTTACCAGTG CTTGATCAGT 5501 GAGGCACCGA TCTCAGCGAT CTGCCTATTT CGTTCGTCCA TAGTGGCCTG 5551 ACTCCCCGTC GTGTAGATCA CTACGATTCG TGAGGGCTTA CCATCAGGCC 5601 CCAGCGCAGC AATGATGCCG CGAGAGCCGC GTTCACCGGC CCCCGATTTG 5651 TCAGCAATGA ACCAGCCAGC AGGGAGGGCC GAGCGAAGAA GTGGTCCTGC 5701 TACTTTGTCC GCCTCCATCC AGTCTATGAG CTGCTGTCGT GATGCTAGAG 5751 TAAGAAGTTC GCCAGTGAGT AGTTTCCGAA GAGTTGTGGC CATTGCTACT 5801 GGCATCGTGG TATCACGCTC GTCGTTCGGT ATGGCTTCGT TCAACTCTGG 5851 TTCCCAGCGG TCAAGCCGGG TCACATGATC ACCCATATTA TGAAGAAATG 5901 CAGTCAGCTC CTTAGGGCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCG 5951 GTGTTGTCGC TCATGGTAAT GGCAGCACTA CACAATTCTC TTACCGTCAT 6001 GCCATCCGTA AGATGCTTTT CCGTGACCGG CGAGTACTCA ACCAAGTCGT 6051 TTTGTGAGTA GTGTATACGG CGACCAAGCT GCTCTTGCCC GGCGTCTATA 6101 CGGGACAACA CCGCGCCACA TAGCAGTACT TTGAAAGTGC TCATCATCGG 6151 GAATCGTTCT TCGGGGCGGA AAGACTCAAG GATCTTGCCG CTATTGAGAT 6201 CCAGTTCGAT ATAGCCCACT CTTGCACCCA GTTGATCTTC AGCATCTTTT 6251 ACTTTCACCA GCGTTTCGGG GTGTGCAAAA ACAGGCAAGC AAAATGCCGC 6301 AAAGAAGGGA ATGAGTGCGA CACGAAAATG TTGGATGCTC ATACTCGTCC 6351 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTACTAGTA CGTCTCTCAA 6401 GGATAAGTAA GTAATATTAA GGTACGGGAG GTATTGGACA GGCCGCAATA 6451 AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT GTGTGAATCG 6501 ATAGTACTAA CATACGCTCT CCATCAAAAC AAAACGAAAC AAAACAAACT 6551 AGCAAAATAG GCTGTCCCCA GTGCAAGTGC AGGTGCCAGA ACATTTCTCT File updated 18-Aug-08.