pGL4.49[luc2P/TCF-LEF/Hygro] Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pGL4.49[luc2P/TCF-LEF/Hygro] Vector sequence reference points: Base pairs 6124 Synthetic polyadenylation signal sequence 105-153 Synthetic poly(A) signal/transcriptional pause region 105-258 Reporter Vector primer3 (RVprimer3) binding region 207-226 TCF-LEF response element 285-404 Minimal promoter 450-480 luc2P reporter gene 513-2288 SV40 late poly(A) region 2328-2549 SV40 early enhancer/promoter 2597-3015 Synthetic hygromycin (HygR) coding region 3040-4077 Synthetic polyadenylation signal sequence 4101-4149 Reporter Vector primer4 (RVprimer4) binding region 4216-4235 ColE1-derived plasmid replication origin 4473 Synthetic beta-lactamase(AmpR) coding region 5264-6124 .. 1 ACTCGTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TACTAGTACG 51 TCTCTCAAGG ATAAGTAAGT AATATTAAGG TACGGGAGGT ATTGGACAGG 101 CCGCAATAAA ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT 151 GTGAATCGAT AGTACTAACA TACGCTCTCC ATCAAAACAA AACGAAACAA 201 AACAAACTAG CAAAATAGGC TGTCCCCAGT GCAAGTGCAG GTGCCAGAAC 251 ATTTCTCTGG CCTAACTGGC CGGTACCTGA GCTCAGATCA AAGGGTTTAA 301 GATCAAAGGG CTTAAGATCA AAGGGTATAA GATCAAAGGG CCTAAGATCA 351 AAGGGACTAA GATCAAAGGG TTTAAGATCA AAGGGCTTAA GATCAAAGGG 401 CCTACTCGAG GATATCAAGA TCTGGCCTCG GCGGCCAAGC TTAGACACTA 451 GAGGGTATAT AATGGAAGCT CGACTTCCAG CTTGGCAATC CGGTACTGTT 501 GGTAAAGCCA CCATGGAAGA TGCCAAAAAC ATTAAGAAGG GCCCAGCGCC 551 ATTCTACCCA CTCGAAGACG GGACCGCCGG CGAGCAGCTG CACAAAGCCA 601 TGAAGCGCTA CGCCCTGGTG CCCGGCACCA TCGCCTTTAC CGACGCACAT 651 ATCGAGGTGG ACATTACCTA CGCCGAGTAC TTCGAGATGA GCGTTCGGCT 701 GGCAGAAGCT ATGAAGCGCT ATGGGCTGAA TACAAACCAT CGGATCGTGG 751 TGTGCAGCGA GAATAGCTTG CAGTTCTTCA TGCCCGTGTT GGGTGCCCTG 801 TTCATCGGTG TGGCTGTGGC CCCAGCTAAC GACATCTACA ACGAGCGCGA 851 GCTGCTGAAC AGCATGGGCA TCAGCCAGCC CACCGTCGTA TTCGTGAGCA 901 AGAAAGGGCT GCAAAAGATC CTCAACGTGC AAAAGAAGCT ACCGATCATA 951 CAAAAGATCA TCATCATGGA TAGCAAGACC GACTACCAGG GCTTCCAAAG 1001 CATGTACACC TTCGTGACTT CCCATTTGCC ACCCGGCTTC AACGAGTACG 1051 ACTTCGTGCC CGAGAGCTTC GACCGGGACA AAACCATCGC CCTGATCATG 1101 AACAGTAGTG GCAGTACCGG ATTGCCCAAG GGCGTAGCCC TACCGCACCG 1151 CACCGCTTGT GTCCGATTCA GTCATGCCCG CGACCCCATC TTCGGCAACC 1201 AGATCATCCC CGACACCGCT ATCCTCAGCG TGGTGCCATT TCACCACGGC 1251 TTCGGCATGT TCACCACGCT GGGCTACTTG ATCTGCGGCT TTCGGGTCGT 1301 GCTCATGTAC CGCTTCGAGG AGGAGCTATT CTTGCGCAGC TTGCAAGACT 1351 ATAAGATTCA ATCTGCCCTG CTGGTGCCCA CACTATTTAG CTTCTTCGCT 1401 AAGAGCACTC TCATCGACAA GTACGACCTA AGCAACTTGC ACGAGATCGC 1451 CAGCGGCGGG GCGCCGCTCA GCAAGGAGGT AGGTGAGGCC GTGGCCAAAC 1501 GCTTCCACCT ACCAGGCATC CGCCAGGGCT ACGGCCTGAC AGAAACAACC 1551 AGCGCCATTC TGATCACCCC CGAAGGGGAC GACAAGCCTG GCGCAGTAGG 1601 CAAGGTGGTG CCCTTCTTCG AGGCTAAGGT GGTGGACTTG GACACCGGTA 1651 AGACACTGGG TGTGAACCAG CGCGGCGAGC TGTGCGTCCG TGGCCCCATG 1701 ATCATGAGCG GCTACGTTAA CAACCCCGAG GCTACAAACG CTCTCATCGA 1751 CAAGGACGGC TGGCTGCACA GCGGCGACAT CGCCTACTGG GACGAGGACG 1801 AGCACTTCTT CATCGTGGAC CGGCTGAAGA GCCTGATCAA ATACAAGGGC 1851 TACCAGGTAG CCCCAGCCGA ACTGGAGAGC ATCCTGCTGC AACACCCCAA 1901 CATCTTCGAC GCCGGGGTCG CCGGCCTGCC CGACGACGAT GCCGGCGAGC 1951 TGCCCGCCGC AGTCGTCGTG CTGGAACACG GTAAAACCAT GACCGAGAAG 2001 GAGATCGTGG ACTATGTGGC CAGCCAGGTT ACAACCGCCA AGAAGCTGCG 2051 CGGTGGTGTT GTGTTCGTGG ACGAGGTGCC TAAAGGACTG ACCGGCAAGT 2101 TGGACGCCCG CAAGATCCGC GAGATTCTCA TTAAGGCCAA GAAGGGCGGC 2151 AAGATCGCCG TGAATTCTCA CGGCTTCCCT CCCGAGGTGG AGGAGCAGGC 2201 CGCCGGCACC CTGCCCATGA GCTGCGCCCA GGAGAGCGGC ATGGATAGAC 2251 ACCCTGCTGC TTGCGCCAGC GCCAGGATCA ACGTCTAAGG CCGCGACTCT 2301 AGAGTCGGGG CGGCCGGCCG CTTCGAGCAG ACATGATAAG ATACATTGAT 2351 GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG 2401 TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA 2451 AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG 2501 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAA 2551 AATCGATAAG GATCCGTTTG CGTATTGGGC GCTCTTCCGC TGATCTGCGC 2601 AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGCTACCT 2651 TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 2701 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA 2751 TTAGTCAGCA ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA 2801 GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA 2851 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC 2901 CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCTG 2951 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 3001 TTTTGCAAAA AGCTCGATTC TTCTGACACT AGCGCCACCA TGAAGAAGCC 3051 CGAACTCACC GCTACCAGCG TTGAAAAATT TCTCATCGAG AAGTTCGACA 3101 GTGTGAGCGA CCTGATGCAG TTGTCGGAGG GCGAAGAGAG CCGAGCCTTC 3151 AGCTTCGATG TCGGCGGACG CGGCTATGTA CTGCGGGTGA ATAGCTGCGC 3201 TGATGGCTTC TACAAAGACC GCTACGTGTA CCGCCACTTC GCCAGCGCTG 3251 CACTACCCAT CCCCGAAGTG TTGGACATCG GCGAGTTCAG CGAGAGCCTG 3301 ACATACTGCA TCAGTAGACG CGCCCAAGGC GTTACTCTCC AAGACCTCCC 3351 CGAAACAGAG CTGCCTGCTG TGTTACAGCC TGTCGCCGAA GCTATGGATG 3401 CTATTGCCGC CGCCGACCTC AGTCAAACCA GCGGCTTCGG CCCATTCGGG 3451 CCCCAAGGCA TCGGCCAGTA CACAACCTGG CGGGATTTCA TTTGCGCCAT 3501 TGCTGATCCC CATGTCTACC ACTGGCAGAC CGTGATGGAC GACACCGTGT 3551 CCGCCAGCGT AGCTCAAGCC CTGGACGAAC TGATGCTGTG GGCCGAAGAC 3601 TGTCCCGAGG TGCGCCACCT CGTCCATGCC GACTTCGGCA GCAACAACGT 3651 CCTGACCGAC AACGGCCGCA TCACCGCCGT AATCGACTGG TCCGAAGCTA 3701 TGTTCGGGGA CAGTCAGTAC GAGGTGGCCA ACATCTTCTT CTGGCGGCCC 3751 TGGCTGGCTT GCATGGAGCA GCAGACTCGC TACTTCGAGC GCCGGCATCC 3801 CGAGCTGGCC GGCAGCCCTC GTCTGCGAGC CTACATGCTG CGCATCGGCC 3851 TGGATCAGCT CTACCAGAGC CTCGTGGACG GCAACTTCGA CGATGCTGCC 3901 TGGGCTCAAG GCCGCTGCGA TGCCATCGTC CGCAGCGGGG CCGGCACCGT 3951 CGGTCGCACA CAAATCGCTC GCCGGAGCGC AGCCGTATGG ACCGACGGCT 4001 GCGTCGAGGT GCTGGCCGAC AGCGGCAACC GCCGGCCCAG TACACGACCG 4051 CGCGCTAAGG AGGTAGGTCG AGTTTAAACT CTAGAACCGG TCATGGCCGC 4101 AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGT 4151 TCGAACTAGA TGCTGTCGAC CGATGCCCTT GAGAGCCTTC AACCCAGTCA 4201 GCTCCTTCCG GTGGGCGCGG GGCATGACTA TCGTCGCCGC ACTTATGACT 4251 GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG CGCTCTTCCG 4301 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG 4351 GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 4401 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 4451 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 4501 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 4551 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC 4601 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 4651 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 4701 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 4751 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 4801 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 4851 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT 4901 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 4951 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 5001 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 5051 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 5101 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 5151 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA 5201 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGCGGCCG CAAATGCTAA 5251 ACCACTGCAG TGGTTACCAG TGCTTGATCA GTGAGGCACC GATCTCAGCG 5301 ATCTGCCTAT TTCGTTCGTC CATAGTGGCC TGACTCCCCG TCGTGTAGAT 5351 CACTACGATT CGTGAGGGCT TACCATCAGG CCCCAGCGCA GCAATGATGC 5401 CGCGAGAGCC GCGTTCACCG GCCCCCGATT TGTCAGCAAT GAACCAGCCA 5451 GCAGGGAGGG CCGAGCGAAG AAGTGGTCCT GCTACTTTGT CCGCCTCCAT 5501 CCAGTCTATG AGCTGCTGTC GTGATGCTAG AGTAAGAAGT TCGCCAGTGA 5551 GTAGTTTCCG AAGAGTTGTG GCCATTGCTA CTGGCATCGT GGTATCACGC 5601 TCGTCGTTCG GTATGGCTTC GTTCAACTCT GGTTCCCAGC GGTCAAGCCG 5651 GGTCACATGA TCACCCATAT TATGAAGAAA TGCAGTCAGC TCCTTAGGGC 5701 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CGGTGTTGTC GCTCATGGTA 5751 ATGGCAGCAC TACACAATTC TCTTACCGTC ATGCCATCCG TAAGATGCTT 5801 TTCCGTGACC GGCGAGTACT CAACCAAGTC GTTTTGTGAG TAGTGTATAC 5851 GGCGACCAAG CTGCTCTTGC CCGGCGTCTA TACGGGACAA CACCGCGCCA 5901 CATAGCAGTA CTTTGAAAGT GCTCATCATC GGGAATCGTT CTTCGGGGCG 5951 GAAAGACTCA AGGATCTTGC CGCTATTGAG ATCCAGTTCG ATATAGCCCA 6001 CTCTTGCACC CAGTTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCG 6051 GGGTGTGCAA AAACAGGCAA GCAAAATGCC GCAAAGAAGG GAATGAGTGC 6101 GACACGAAAA TGTTGGATGC TCAT Sequence file prepared 6-June-2012.