pFN24K (HaloTag(R)) CMVd3 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN24K (HaloTag(R)) CMVd3 Flexi(R) Vector sequence reference points: Base pairs 4128 CMVd3 intermediate early enhancer/promoter 1-66 T7 RNA polymerase promoter (-17 to +3) 77-96 SP6 RNA polymerase promoter (-17 to +3) 101-120 HaloTag(R) 7 protein coding region 136-1026 TEV region 1039-1059 SgfI region 1066-1073 Barnase coding region 1097-1432 PmeI region 1434-1441 SV40 late polyadenylation signal 1593-1814 Kanamycin resistance (Kanr) coding region 2122-2916 ColE1-derived plasmid origin of replication 3085-3121 cer region (for E. coli XerCD recombinase) 3792-4077 .. 1 ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTGGTTT 51 AGTGAACCGT CAGATCACTA GAAGCTTAAT ACGACTCACT ATAGGGTACG 101 ATTTAGGTGA CACTATAGAA TAAGGAGAAT TCATCATGGC AGAAATCGGT 151 ACTGGCTTTC CATTCGACCC CCATTATGTG GAAGTCCTGG GCGAGCGCAT 201 GCACTACGTC GATGTTGGTC CGCGCGATGG CACCCCTGTG CTGTTCCTGC 251 ACGGTAACCC GACCTCCTCC TACGTGTGGC GCAACATCAT CCCGCATGTT 301 GCACCGACCC ATCGCTGCAT TGCTCCAGAC CTGATCGGTA TGGGCAAATC 351 CGACAAACCA GACCTGGGTT ATTTCTTCGA CGACCACGTC CGCTTCATGG 401 ATGCCTTCAT CGAAGCCCTG GGTCTGGAAG AGGTCGTCCT GGTCATTCAC 451 GACTGGGGCT CCGCTCTGGG TTTCCACTGG GCCAAGCGCA ATCCAGAGCG 501 CGTCAAAGGT ATTGCATTTA TGGAGTTCAT CCGCCCTATC CCGACCTGGG 551 ACGAATGGCC AGAATTTGCC CGCGAGACCT TCCAGGCCTT CCGCACCACC 601 GACGTCGGCC GCAAGCTGAT CATCGATCAG AACGTTTTTA TCGAGGGTAC 651 GCTGCCGATG GGTGTCGTCC GCCCGCTGAC TGAAGTCGAG ATGGACCATT 701 ACCGCGAGCC GTTCCTGAAT CCTGTTGACC GCGAGCCACT GTGGCGCTTC 751 CCAAACGAGC TGCCAATCGC CGGTGAGCCA GCGAACATCG TCGCGCTGGT 801 CGAAGAATAC ATGGACTGGC TGCACCAGTC CCCTGTCCCG AAGCTGCTGT 851 TCTGGGGCAC CCCAGGCGTT CTGATCCCAC CGGCCGAAGC CGCTCGCCTG 901 GCCAAAAGCC TGCCTAACTG CAAGGCTGTG GACATCGGCC CGGGTCTGAA 951 TCTGCTGCAA GAAGACAACC CGGACCTGAT CGGCAGCGAG ATCGCGCGCT 1001 GGCTGTCGAC GCTCGAGATT TCCGGCGAGC CAACCACTGA GGATCTGTAC 1051 TTTCAGAGCG ATAACGCGAT CGCCATGGAA TAAGTAAGGA ATCCACATGG 1101 CACAGGTTAT CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT 1151 CATAAGCTAC CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG 1201 CTGGGTGGCA TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA 1251 TCGGCGGAGA CATCTTCTCA AACAGGGAAG GCAAACTCCC GGGCAAAAGC 1301 GGACGAACAT GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA 1351 TTCAGACCGG ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG 1401 ACCATTATCA GACCTTTACA AAAATCAGAT AATGTTTAAA CGAATTCGGG 1451 CTCGGTACCC GGGGATCCTC TAGAGTCGAC CTGCAGGCAT GCAAGCTGAT 1501 CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC 1551 TGAGCAATAA CTAGCATAAC CCCTTGGGGC GGCCGCTTCG AGCAGACATG 1601 ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA 1651 AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA 1701 TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG 1751 TTTCAGGTTC AGGGGGAGAT GTGGGAGGTT TTTTTAAGCA AGTAAAACCT 1801 CTACAAATGT GGTAAAATCG AATTCTAATG GATCCTCTTT GCGCTTGCGT 1851 TTTCCCTTGT CCAGATAGCC CAGTAGCTGA CATTCATCCG GGGTCAGCAC 1901 CGTTTCTGCG GACTGGCTTT CTACGTGTTC CGCTTCCTTT AGCAGCCCTT 1951 GCGCCCTGAG TGCTTGCGGC AGCGTGAGCT TCAAAAGAAT TGCCAGCTGG 2001 GGCGCCCTCT GGTAAGGTTG GGAAGCCCTG CAAAGTAAAC TGGATGGCTT 2051 TCTTGCCGCC AAGGATCTGA TGGCGCAGGG GATCAAGATC TGATCAAGAG 2101 ACAGGATGAC GGTCGTTTCG CATGCTTGAA CAAGATGGAT TGCACGCAGG 2151 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC 2201 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 2251 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT 2301 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT 2351 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 2401 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 2451 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG 2501 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 2551 GCACGCACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA 2601 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC 2651 GTATGCCGGA TGGTGAGGAT CTCGTCGTGA CTCATGGCGA TGCCTGCTTG 2701 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 2751 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG 2801 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 2851 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT 2901 TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC 2951 GACGCCCAAC CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 3001 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 3051 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 3101 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 3151 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 3201 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 3251 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 3301 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 3351 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 3401 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 3451 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 3501 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 3551 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 3601 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 3651 TTACGCGCAG AAAAAAAGGA TTTCAAGAAG ATCCTTTGAT CTTTTCTACG 3701 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 3751 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTATAG TCCGGAAATA 3801 CAGGAACGCA CGCTGGATGG CCCTTCGCTG GGATGGTGAA ACCATGAAAA 3851 ATGGCAGCTT CAGTGGATTA AGTGGGGGTA ATGTGGCCTG TACCCTCTGG 3901 TTGCATAGGT ATTCATACGG TTAAAATTTA TCAGGCGCGA TTGCGGCAGT 3951 TTTTCGGGTG GTTTGTTGCC ATTTTTACCT GTCTGCTGCC GTGATCGCGC 4001 TGAACGCGTT TTAGCGGTGC GTACAATTAA GGGATTATGG TAAATCCACT 4051 TACTGTCTGC CCTCGTAGCC ATCGAGATAA ACCGCAGTAC TCCGGCCACG 4101 ATGCGTCCGG CGTAGAGGAT CGAGATCT File updated 28-Apr-08.