pFN19K (HaloTag(R) 7) T7 SP6 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN19K (HaloTag(R) 7) T7 SP6 Flexi(R) Vector sequence reference points: Base pairs 4421 T7 RNA polymerase promoter (-17 to +3) 21-40 SP6 RNA polymerase promoter (-17 to +3) 45-64 HaloTag(R) 7 protein coding region 80-970 TEV protease site 983-1003 Sgf I site 1010-1017 Barnase coding region 1041-1376 Pme I site 1378-1385 Synthetic poly(A) region 1516-1543 T7 terminator 1544-1591 Kanamycin resistance (Kanr) coding region 1972-2766 ColE1-derived plasmid origin of replication 2935-2971 cer site (site for E. coli XerCD recombinase) 3642-3927 rrnB transcription terminator 3978-4379 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG TACGATTTAG 51 GTGACACTAT AGAATAAGGA GAATTCATCA TGGCAGAAAT CGGTACTGGC 101 TTTCCATTCG ACCCCCATTA TGTGGAAGTC CTGGGCGAGC GCATGCACTA 151 CGTCGATGTT GGTCCGCGCG ATGGCACCCC TGTGCTGTTC CTGCACGGTA 201 ACCCGACCTC CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG 251 ACCCATCGCT GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA 301 ACCAGACCTG GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT 351 TCATCGAAGC CCTGGGTCTG GAAGAGGTCG TCCTGGTCAT TCACGACTGG 401 GGCTCCGCTC TGGGTTTCCA CTGGGCCAAG CGCAATCCAG AGCGCGTCAA 451 AGGTATTGCA TTTATGGAGT TCATCCGCCC TATCCCGACC TGGGACGAAT 501 GGCCAGAATT TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC 551 GGCCGCAAGC TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC 601 GATGGGTGTC GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG 651 AGCCGTTCCT GAATCCTGTT GACCGCGAGC CACTGTGGCG CTTCCCAAAC 701 GAGCTGCCAA TCGCCGGTGA GCCAGCGAAC ATCGTCGCGC TGGTCGAAGA 751 ATACATGGAC TGGCTGCACC AGTCCCCTGT CCCGAAGCTG CTGTTCTGGG 801 GCACCCCAGG CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA 851 AGCCTGCCTA ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT 901 GCAAGAAGAC AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT 951 CGACGCTCGA GATTTCCGGC GAGCCAACCA CTGAGGATCT GTACTTTCAG 1001 AGCGATAACG CGATCGCCAT GGAATAAGTA AGGAATCCAC ATGGCACAGG 1051 TTATCAACAC GTTTGACGGG GTTGCGGATT ATCTTCAGAC ATATCATAAG 1101 CTACCTGATA ATTACATTAC AAAATCAGAA GCACAAGCCC TCGGCTGGGT 1151 GGCATCAAAA GGGAACCTTG CAGACGTCGC TCCGGGGAAA AGCATCGGCG 1201 GAGACATCTT CTCAAACAGG GAAGGCAAAC TCCCGGGCAA AAGCGGACGA 1251 ACATGGCGTG AAGCGGATAT TAACTATACA TCAGGCTTCA GAAATTCAGA 1301 CCGGATTCTT TACTCAAGCG ACTGGCTGAT TTACAAAACA ACGGACCATT 1351 ATCAGACCTT TACAAAAATC AGATAATGTT TAAACGAATT CGGGCTCGGT 1401 ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TGATCCGGCT 1451 GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA 1501 ATAACTAGCA TAACCAAAAA AAAAAAAAAA AAAAAAAAAA AAACTAGCAT 1551 AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA 1601 GGAACTATAT CCGGTTCGCT TGCTGTCCAT AAAACCGCCC AGTCTAGCTA 1651 TCGCCATGTA AGCCCACTGC AAGCTACCTG CTTTCTCTTT GCGCTTGCGT 1701 TTTCCCTTGT CCAGATAGCC CAGTAGCTGA CATTCATCCG GGGTCAGCAC 1751 CGTTTCTGCG GACTGGCTTT CTACGTGTTC CGCTTCCTTT AGCAGCCCTT 1801 GCGCCCTGAG TGCTTGCGGC AGCGTGAGCT TCAAAAGAAT TGCCAGCTGG 1851 GGCGCCCTCT GGTAAGGTTG GGAAGCCCTG CAAAGTAAAC TGGATGGCTT 1901 TCTTGCCGCC AAGGATCTGA TGGCGCAGGG GATCAAGATC TGATCAAGAG 1951 ACAGGATGAC GGTCGTTTCG CATGCTTGAA CAAGATGGAT TGCACGCAGG 2001 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC 2051 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG 2101 CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT 2151 GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT 2201 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA 2251 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC 2301 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG 2351 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA 2401 GCACGCACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA 2451 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC 2501 GTATGCCGGA TGGTGAGGAT CTCGTCGTGA CTCATGGCGA TGCCTGCTTG 2551 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG 2601 CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG 2651 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT 2701 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT 2751 TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC 2801 GACGCCCAAC CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 2851 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA 2901 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 2951 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 3001 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 3051 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 3101 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 3151 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC 3201 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 3251 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 3301 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 3351 GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 3401 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 3451 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 3501 TTACGCGCAG AAAAAAAGGA TTTCAAGAAG ATCCTTTGAT CTTTTCTACG 3551 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 3601 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTATAG TCCGGAAATA 3651 CAGGAACGCA CGCTGGATGG CCCTTCGCTG GGATGGTGAA ACCATGAAAA 3701 ATGGCAGCTT CAGTGGATTA AGTGGGGGTA ATGTGGCCTG TACCCTCTGG 3751 TTGCATAGGT ATTCATACGG TTAAAATTTA TCAGGCGCGA TTGCGGCAGT 3801 TTTTCGGGTG GTTTGTTGCC ATTTTTACCT GTCTGCTGCC GTGATCGCGC 3851 TGAACGCGTT TTAGCGGTGC GTACAATTAA GGGATTATGG TAAATCCACT 3901 TACTGTCTGC CCTCGTAGCC ATCGAGATAA ACCGCAGTAC TCCGGCCACG 3951 ATGCGTCCGG CGTAGAGGAT CGAGATCTTT TCAGCCTGAT ACAGATTAAA 4001 TCAGAACGCA GAAGCGGTCT GATAAAACAG AATTTGCCTG GCGGCAGTAG 4051 CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG AAACGCCGTA 4101 GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG 4151 GCATCAAATA AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA 4201 TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA TCCGCCGGGA 4251 GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC GGGCAGGACG 4301 CCCGCCATAA ACTGCCAGGC ATCAAATTAA GCAGAAGGCC ATCCTGACGG 4351 ATGGCCTTTT TGCGTTTCTA CAAACTCTTT TGTTTATTTT TCTAAATACA 4401 TTCAAATATG TATCCGCTCA T Sequence file prepared 17-Apr-08.