pFN19A (HaloTag(R) 7) T7 SP6 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN19A (HaloTag(R) 7) T7 SP6 Flexi(R) Vector sequence reference points: Base pairs 4428 T7 RNA polymerase promoter (-17 to +3) 21-40 SP6 RNA polymerase promoter (-17 to +3) 45-64 HaloTag(R) 7 protein coding region 80-970 TEV protease site 983-1003 Sgf I site 1010-1017 Barnase coding region 1041-1376 Pme I site 1378-1385 Synthetic poly(A) region 1516-1545 T7 terminator 1546-1593 Beta-lactamase (Ampr) coding region 1927-2787 ColE1-derived plasmid origin of replication 2942-2978 cer site (site for E. coli XerCD recombinase) 3649-3934 rrnB transcription terminator 3985-4386 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG TACGATTTAG 51 GTGACACTAT AGAATAAGGA GAATTCATCA TGGCAGAAAT CGGTACTGGC 101 TTTCCATTCG ACCCCCATTA TGTGGAAGTC CTGGGCGAGC GCATGCACTA 151 CGTCGATGTT GGTCCGCGCG ATGGCACCCC TGTGCTGTTC CTGCACGGTA 201 ACCCGACCTC CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG 251 ACCCATCGCT GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA 301 ACCAGACCTG GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT 351 TCATCGAAGC CCTGGGTCTG GAAGAGGTCG TCCTGGTCAT TCACGACTGG 401 GGCTCCGCTC TGGGTTTCCA CTGGGCCAAG CGCAATCCAG AGCGCGTCAA 451 AGGTATTGCA TTTATGGAGT TCATCCGCCC TATCCCGACC TGGGACGAAT 501 GGCCAGAATT TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC 551 GGCCGCAAGC TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC 601 GATGGGTGTC GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG 651 AGCCGTTCCT GAATCCTGTT GACCGCGAGC CACTGTGGCG CTTCCCAAAC 701 GAGCTGCCAA TCGCCGGTGA GCCAGCGAAC ATCGTCGCGC TGGTCGAAGA 751 ATACATGGAC TGGCTGCACC AGTCCCCTGT CCCGAAGCTG CTGTTCTGGG 801 GCACCCCAGG CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA 851 AGCCTGCCTA ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT 901 GCAAGAAGAC AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT 951 CGACGCTCGA GATTTCCGGC GAGCCAACCA CTGAGGATCT GTACTTTCAG 1001 AGCGATAACG CGATCGCCAT GGAATAAGTA AGGAATCCAC ATGGCACAGG 1051 TTATCAACAC GTTTGACGGG GTTGCGGATT ATCTTCAGAC ATATCATAAG 1101 CTACCTGATA ATTACATTAC AAAATCAGAA GCACAAGCCC TCGGCTGGGT 1151 GGCATCAAAA GGGAACCTTG CAGACGTCGC TCCGGGGAAA AGCATCGGCG 1201 GAGACATCTT CTCAAACAGG GAAGGCAAAC TCCCGGGCAA AAGCGGACGA 1251 ACATGGCGTG AAGCGGATAT TAACTATACA TCAGGCTTCA GAAATTCAGA 1301 CCGGATTCTT TACTCAAGCG ACTGGCTGAT TTACAAAACA ACGGACCATT 1351 ATCAGACCTT TACAAAAATC AGATAATGTT TAAACGAATT CGGGCTCGGT 1401 ACCCGGGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TGATCCGGCT 1451 GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA 1501 ATAACTAGCA TAACCAAAAA AAAAAAAAAA AAAAAAAAAA AAAAACTAGC 1551 ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG 1601 GAGGAACTAT ATCCGGTTCG CTTGCTGTCC ATAAAACCGC CCAGTCTAGC 1651 TATCGCCATG TAAGCCCACT GCAAGCTACC TGCTTTCTCT TTGCGCTTGC 1701 GTTTTCCCTT GTCCAGATAG CCCAGTAGCT GACATTCATC CGGGGTCAGC 1751 ACCGTTTCTG CGGACTGGCT TTCTACGTAA TGGTTTCTTA GACGTCAGGT 1801 GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA 1851 AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC 1901 TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC 1951 GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC 2001 AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 2051 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT 2101 CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTCAAAG TTCTGCTATG 2151 TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC 2201 GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA 2251 AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT 2301 AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACTATCGGAG 2351 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT 2401 CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA 2451 GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT 2501 TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG 2551 ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC 2601 TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG 2651 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT 2701 ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT 2751 CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAATTC GAAATGACCG 2801 ACCAAGCGAC GCCCAACCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 2851 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC 2901 CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 2951 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 3001 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA 3051 AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 3101 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 3151 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG 3201 CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 3251 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 3301 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC 3351 TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT 3401 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 3451 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG 3501 CAGCAGATTA CGCGCAGAAA AAAAGGATTT CAAGAAGATC CTTTGATCTT 3551 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 3601 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTATAGTCC 3651 GGAAATACAG GAACGCACGC TGGATGGCCC TTCGCTGGGA TGGTGAAACC 3701 ATGAAAAATG GCAGCTTCAG TGGATTAAGT GGGGGTAATG TGGCCTGTAC 3751 CCTCTGGTTG CATAGGTATT CATACGGTTA AAATTTATCA GGCGCGATTG 3801 CGGCAGTTTT TCGGGTGGTT TGTTGCCATT TTTACCTGTC TGCTGCCGTG 3851 ATCGCGCTGA ACGCGTTTTA GCGGTGCGTA CAATTAAGGG ATTATGGTAA 3901 ATCCACTTAC TGTCTGCCCT CGTAGCCATC GAGATAAACC GCAGTACTCC 3951 GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTTTTCA GCCTGATACA 4001 GATTAAATCA GAACGCAGAA GCGGTCTGAT AAAACAGAAT TTGCCTGGCG 4051 GCAGTAGCGC GGTGGTCCCA CCTGACCCCA TGCCGAACTC AGAAGTGAAA 4101 CGCCGTAGCG CCGATGGTAG TGTGGGGTCT CCCCATGCGA GAGTAGGGAA 4151 CTGCCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA CTGGGCCTTT 4201 CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCCTGAGTA GGACAAATCC 4251 GCCGGGAGCG GATTTGAACG TTGCGAAGCA ACGGCCCGGA GGGTGGCGGG 4301 CAGGACGCCC GCCATAAACT GCCAGGCATC AAATTAAGCA GAAGGCCATC 4351 CTGACGGATG GCCTTTTTGC GTTTCTACAA ACTCTTTTGT TTATTTTTCT 4401 AAATACATTC AAATATGTAT CCGCTCAT Sequence file prepared 17-Apr-08.