pFN18K (HaloTag(R) 7) T7 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN18K (HaloTag(R) 7) T7 Flexi(R) Vector sequence reference points: Base pairs 4372 T7 RNA polymerase promoter (-17 to +3) 21-40 HaloTag(R) 7 protein coding region 70-960 TEV protease site 973-993 Sgf I site 1000-1007 Barnase coding region 1031-1366 Pme I site 1368-1375 T7 terminator 1495-1542 Kanamycin resistance (Kanr) coding region 1923-2717 ColE1-derived plasmid origin of replication 2886-2922 cer site (site for E. coli XerCD recombinase) 3593-3878 rrnB transcription terminator 3929-4330 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG TATTTTAACT 51 TTACTAAGGA GAATTCATCA TGGCAGAAAT CGGTACTGGC TTTCCATTCG 101 ACCCCCATTA TGTGGAAGTC CTGGGCGAGC GCATGCACTA CGTCGATGTT 151 GGTCCGCGCG ATGGCACCCC TGTGCTGTTC CTGCACGGTA ACCCGACCTC 201 CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG ACCCATCGCT 251 GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG 301 GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC 351 CCTGGGTCTG GAAGAGGTCG TCCTGGTCAT TCACGACTGG GGCTCCGCTC 401 TGGGTTTCCA CTGGGCCAAG CGCAATCCAG AGCGCGTCAA AGGTATTGCA 451 TTTATGGAGT TCATCCGCCC TATCCCGACC TGGGACGAAT GGCCAGAATT 501 TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC GGCCGCAAGC 551 TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC 601 GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT 651 GAATCCTGTT GACCGCGAGC CACTGTGGCG CTTCCCAAAC GAGCTGCCAA 701 TCGCCGGTGA GCCAGCGAAC ATCGTCGCGC TGGTCGAAGA ATACATGGAC 751 TGGCTGCACC AGTCCCCTGT CCCGAAGCTG CTGTTCTGGG GCACCCCAGG 801 CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA AGCCTGCCTA 851 ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC 901 AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT CGACGCTCGA 951 GATTTCCGGC GAGCCAACCA CTGAGGATCT GTACTTTCAG AGCGATAACG 1001 CGATCGCCAT GGAATAAGTA AGGAATCCAC ATGGCACAGG TTATCAACAC 1051 GTTTGACGGG GTTGCGGATT ATCTTCAGAC ATATCATAAG CTACCTGATA 1101 ATTACATTAC AAAATCAGAA GCACAAGCCC TCGGCTGGGT GGCATCAAAA 1151 GGGAACCTTG CAGACGTCGC TCCGGGGAAA AGCATCGGCG GAGACATCTT 1201 CTCAAACAGG GAAGGCAAAC TCCCGGGCAA AAGCGGACGA ACATGGCGTG 1251 AAGCGGATAT TAACTATACA TCAGGCTTCA GAAATTCAGA CCGGATTCTT 1301 TACTCAAGCG ACTGGCTGAT TTACAAAACA ACGGACCATT ATCAGACCTT 1351 TACAAAAATC AGATAATGTT TAAACGAATT CGGGCTCGGT ACCCGGGGAT 1401 CCTCTAGAGT CGACCTGCAG GCATGCAAGC TGATCCGGCT GCTAACAAAG 1451 CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA 1501 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG 1551 AGGAACTATA TCCGGTTCGC TTGCTGTCCA TAAAACCGCC CAGTCTAGCT 1601 ATCGCCATGT AAGCCCACTG CAAGCTACCT GCTTTCTCTT TGCGCTTGCG 1651 TTTTCCCTTG TCCAGATAGC CCAGTAGCTG ACATTCATCC GGGGTCAGCA 1701 CCGTTTCTGC GGACTGGCTT TCTACGTGTT CCGCTTCCTT TAGCAGCCCT 1751 TGCGCCCTGA GTGCTTGCGG CAGCGTGAGC TTCAAAAGAA TTGCCAGCTG 1801 GGGCGCCCTC TGGTAAGGTT GGGAAGCCCT GCAAAGTAAA CTGGATGGCT 1851 TTCTTGCCGC CAAGGATCTG ATGGCGCAGG GGATCAAGAT CTGATCAAGA 1901 GACAGGATGA CGGTCGTTTC GCATGCTTGA ACAAGATGGA TTGCACGCAG 1951 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA 2001 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG 2051 GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC 2101 TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT 2151 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT 2201 ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG 2251 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT 2301 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG 2351 AGCACGCACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG 2401 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG 2451 CGTATGCCGG ATGGTGAGGA TCTCGTCGTG ACTCATGGCG ATGCCTGCTT 2501 GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG 2551 GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT 2601 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT 2651 TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC 2701 TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG 2751 CGACGCCCAA CCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA 2801 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA 2851 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 2901 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC 2951 GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC 3001 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 3051 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 3101 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 3151 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 3201 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA 3251 ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG 3301 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC 3351 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 3401 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 3451 ATTACGCGCA GAAAAAAAGG ATTTCAAGAA GATCCTTTGA TCTTTTCTAC 3501 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA 3551 TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTATA GTCCGGAAAT 3601 ACAGGAACGC ACGCTGGATG GCCCTTCGCT GGGATGGTGA AACCATGAAA 3651 AATGGCAGCT TCAGTGGATT AAGTGGGGGT AATGTGGCCT GTACCCTCTG 3701 GTTGCATAGG TATTCATACG GTTAAAATTT ATCAGGCGCG ATTGCGGCAG 3751 TTTTTCGGGT GGTTTGTTGC CATTTTTACC TGTCTGCTGC CGTGATCGCG 3801 CTGAACGCGT TTTAGCGGTG CGTACAATTA AGGGATTATG GTAAATCCAC 3851 TTACTGTCTG CCCTCGTAGC CATCGAGATA AACCGCAGTA CTCCGGCCAC 3901 GATGCGTCCG GCGTAGAGGA TCGAGATCTT TTCAGCCTGA TACAGATTAA 3951 ATCAGAACGC AGAAGCGGTC TGATAAAACA GAATTTGCCT GGCGGCAGTA 4001 GCGCGGTGGT CCCACCTGAC CCCATGCCGA ACTCAGAAGT GAAACGCCGT 4051 AGCGCCGATG GTAGTGTGGG GTCTCCCCAT GCGAGAGTAG GGAACTGCCA 4101 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT 4151 ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG AGTAGGACAA ATCCGCCGGG 4201 AGCGGATTTG AACGTTGCGA AGCAACGGCC CGGAGGGTGG CGGGCAGGAC 4251 GCCCGCCATA AACTGCCAGG CATCAAATTA AGCAGAAGGC CATCCTGACG 4301 GATGGCCTTT TTGCGTTTCT ACAAACTCTT TTGTTTATTT TTCTAAATAC 4351 ATTCAAATAT GTATCCGCTC AT Sequence file prepared 14-Apr-08.