pFN18A (HaloTag(R) 7) T7 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFN18A (HaloTag(R) 7) T7 Flexi(R) Vector sequence reference points: Base pairs 4377 T7 RNA polymerase promoter (-17 to +3) 21-40 HaloTag(R) 7 protein coding region 70-960 TEV protease site 973-993 Sgf I site 1000-1007 Barnase coding region 1031-1366 Pme I site 1368-1375 T7 terminator 1495-1542 Beta-lactamase coding region 1876-2736 ColE1-derived plasmid origin of replication 2891-2927 cer site (site for E. coli XerCD recombinase) 3598-3883 rrnB transcription terminator 3934-4335 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG TATTTTAACT 51 TTACTAAGGA GAATTCATCA TGGCAGAAAT CGGTACTGGC TTTCCATTCG 101 ACCCCCATTA TGTGGAAGTC CTGGGCGAGC GCATGCACTA CGTCGATGTT 151 GGTCCGCGCG ATGGCACCCC TGTGCTGTTC CTGCACGGTA ACCCGACCTC 201 CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG ACCCATCGCT 251 GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG 301 GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC 351 CCTGGGTCTG GAAGAGGTCG TCCTGGTCAT TCACGACTGG GGCTCCGCTC 401 TGGGTTTCCA CTGGGCCAAG CGCAATCCAG AGCGCGTCAA AGGTATTGCA 451 TTTATGGAGT TCATCCGCCC TATCCCGACC TGGGACGAAT GGCCAGAATT 501 TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC GGCCGCAAGC 551 TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC 601 GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT 651 GAATCCTGTT GACCGCGAGC CACTGTGGCG CTTCCCAAAC GAGCTGCCAA 701 TCGCCGGTGA GCCAGCGAAC ATCGTCGCGC TGGTCGAAGA ATACATGGAC 751 TGGCTGCACC AGTCCCCTGT CCCGAAGCTG CTGTTCTGGG GCACCCCAGG 801 CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA AGCCTGCCTA 851 ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC 901 AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT CGACGCTCGA 951 GATTTCCGGC GAGCCAACCA CTGAGGATCT GTACTTTCAG AGCGATAACG 1001 CGATCGCCAT GGAATAAGTA AGGAATCCAC ATGGCACAGG TTATCAACAC 1051 GTTTGACGGG GTTGCGGATT ATCTTCAGAC ATATCATAAG CTACCTGATA 1101 ATTACATTAC AAAATCAGAA GCACAAGCCC TCGGCTGGGT GGCATCAAAA 1151 GGGAACCTTG CAGACGTCGC TCCGGGGAAA AGCATCGGCG GAGACATCTT 1201 CTCAAACAGG GAAGGCAAAC TCCCGGGCAA AAGCGGACGA ACATGGCGTG 1251 AAGCGGATAT TAACTATACA TCAGGCTTCA GAAATTCAGA CCGGATTCTT 1301 TACTCAAGCG ACTGGCTGAT TTACAAAACA ACGGACCATT ATCAGACCTT 1351 TACAAAAATC AGATAATGTT TAAACGAATT CGGGCTCGGT ACCCGGGGAT 1401 CCTCTAGAGT CGACCTGCAG GCATGCAAGC TGATCCGGCT GCTAACAAAG 1451 CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA 1501 TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG 1551 AGGAACTATA TCCGGTTCGC TTGCTGTCCA TAAAACCGCC CAGTCTAGCT 1601 ATCGCCATGT AAGCCCACTG CAAGCTACCT GCTTTCTCTT TGCGCTTGCG 1651 TTTTCCCTTG TCCAGATAGC CCAGTAGCTG ACATTCATCC GGGGTCAGCA 1701 CCGTTTCTGC GGACTGGCTT TCTACGTAAT GGTTTCTTAG ACGTCAGGTG 1751 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA 1801 ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT 1851 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG 1901 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA 1951 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 2001 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC 2051 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTCAAAGT TCTGCTATGT 2101 GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG 2151 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA 2201 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA 2251 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CTATCGGAGG 2301 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC 2351 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG 2401 CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT 2451 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA 2501 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT 2551 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 2601 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA 2651 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC 2701 GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAATTCG AAATGACCGA 2751 CCAAGCGACG CCCAACCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 2801 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 2851 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 2901 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 2951 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 3001 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 3051 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 3101 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 3151 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 3201 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 3251 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 3301 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC 3351 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 3401 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 3451 AGCAGATTAC GCGCAGAAAA AAAGGATTTC AAGAAGATCC TTTGATCTTT 3501 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 3551 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTATAGTCCG 3601 GAAATACAGG AACGCACGCT GGATGGCCCT TCGCTGGGAT GGTGAAACCA 3651 TGAAAAATGG CAGCTTCAGT GGATTAAGTG GGGGTAATGT GGCCTGTACC 3701 CTCTGGTTGC ATAGGTATTC ATACGGTTAA AATTTATCAG GCGCGATTGC 3751 GGCAGTTTTT CGGGTGGTTT GTTGCCATTT TTACCTGTCT GCTGCCGTGA 3801 TCGCGCTGAA CGCGTTTTAG CGGTGCGTAC AATTAAGGGA TTATGGTAAA 3851 TCCACTTACT GTCTGCCCTC GTAGCCATCG AGATAAACCG CAGTACTCCG 3901 GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTTTTCAG CCTGATACAG 3951 ATTAAATCAG AACGCAGAAG CGGTCTGATA AAACAGAATT TGCCTGGCGG 4001 CAGTAGCGCG GTGGTCCCAC CTGACCCCAT GCCGAACTCA GAAGTGAAAC 4051 GCCGTAGCGC CGATGGTAGT GTGGGGTCTC CCCATGCGAG AGTAGGGAAC 4101 TGCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC 4151 GTTTTATCTG TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG 4201 CCGGGAGCGG ATTTGAACGT TGCGAAGCAA CGGCCCGGAG GGTGGCGGGC 4251 AGGACGCCCG CCATAAACTG CCAGGCATCA AATTAAGCAG AAGGCCATCC 4301 TGACGGATGG CCTTTTTGCG TTTCTACAAA CTCTTTTGTT TATTTTTCTA 4351 AATACATTCA AATATGTATC CGCTCAT Sequence file prepared 14-Apr-08.