pFC27A HaloTag(R) CMV-neo Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC27A HaloTag(R) CMV-neo Flexi(R) Vector sequence reference points: Base pairs 6524 CMV immediate early enhancer/promoter 1-742 Chimeric intron 857-989 T7 RNA polymerase promoter (-17 to +3) 1033-1052 Sgf I site 1056-1063 Barnase coding region 1087-1422 EcoICRI region 1442-1447 HaloTag linker 1447-1491 TEV protease recognition region 1462-1482 HaloTag 7 C terminal region 1492-2382 SV40 late poly(A) region 2516-2737 SV40 enhancer and early promoter 2836-3254 Neomycin phosphotransferase gene 3299-4093 Synthetic polyadenylation region 4157-4205 Beta-lactamase coding region 4466-5326 ColE1-derived plasmid origin of replication 5481-5517 .. 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCGAT CGCCATGGAA TAAGTAAGGA ATCCACATGG CACAGGTTAT 1101 CAACACGTTT GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC 1151 CTGATAATTA CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA 1201 TCAAAAGGGA ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA 1251 CATCTTCTCA AACAGGGAAG GCAAACTCCC GGGCAAAAGC GGACGAACAT 1301 GGCGTGAAGC GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG 1351 ATTCTTTACT CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA 1401 GACCTTTACA AAAATCAGAT AATGTTTAAT GACCCCGTGT CGAGCTCTCG 1451 AGCCAACCAC TGAGGATCTG TACTTTCAGA GCGATAACGA TGGATCCGAA 1501 ATCGGTACTG GCTTTCCATT CGACCCCCAT TATGTGGAAG TCCTGGGCGA 1551 GCGCATGCAC TACGTCGATG TTGGTCCGCG CGATGGCACC CCTGTGCTGT 1601 TCCTGCACGG TAACCCGACC TCCTCCTACG TGTGGCGCAA CATCATCCCG 1651 CATGTTGCAC CGACCCATCG CTGCATTGCT CCAGACCTGA TCGGTATGGG 1701 CAAATCCGAC AAACCAGACC TGGGTTATTT CTTCGACGAC CACGTCCGCT 1751 TCATGGATGC CTTCATCGAA GCCCTGGGTC TGGAAGAGGT CGTCCTGGTC 1801 ATTCACGACT GGGGCTCCGC TCTGGGTTTC CACTGGGCCA AGCGCAATCC 1851 AGAGCGCGTC AAAGGTATTG CATTTATGGA GTTCATCCGC CCTATCCCGA 1901 CCTGGGACGA ATGGCCAGAA TTTGCCCGCG AGACCTTCCA GGCCTTCCGC 1951 ACCACCGACG TCGGCCGCAA GCTGATCATC GATCAGAACG TTTTTATCGA 2001 GGGTACGCTG CCGATGGGTG TCGTCCGCCC GCTGACTGAA GTCGAGATGG 2051 ACCATTACCG CGAGCCGTTC CTGAATCCTG TTGACCGCGA GCCACTGTGG 2101 CGCTTCCCAA ACGAGCTGCC AATCGCCGGT GAGCCAGCGA ACATCGTCGC 2151 GCTGGTCGAA GAATACATGG ACTGGCTGCA CCAGTCCCCT GTCCCGAAGC 2201 TGCTGTTCTG GGGCACCCCA GGCGTTCTGA TCCCACCGGC CGAAGCCGCT 2251 CGCCTGGCCA AAAGCCTGCC TAACTGCAAG GCTGTGGACA TCGGCCCGGG 2301 TCTGAATCTG CTGCAAGAAG ACAACCCGGA CCTGATCGGC AGCGAGATCG 2351 CGCGCTGGCT GTCTACTCTG GAGATTTCCG GTTAATAGAA TTCTAGAGTC 2401 GACCTGCAGG CATGCAAGCT GATCCGGCTG CTAACAAAGC CCGAAAGGAA 2451 GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG 2501 GGCGGCCGCT TCGAGCAGAC ATGATAAGAT ACATTGATGA GTTTGGACAA 2551 ACCACAACTA GAATGCAGTG AAAAAAATGC TTTATTTGTG AAATTTGTGA 2601 TGCTATTGCT TTATTTGTAA CCATTATAAG CTGCAATAAA CAAGTTAACA 2651 ACAACAATTG CATTCATTTT ATGTTTCAGG TTCAGGGGGA GATGTGGGAG 2701 GTTTTTTTAA GCAAGTAAAA CCTCTACAAA TGTGGTAAAA TCGAATTTTA 2751 ACAAAATATT AACGCTTACA ATTTCCTGAT GCGGTATTTT CTCCTTACGC 2801 ATCTGTGCGG TATTTCACAC CGCATACGCG GATCTGCGCA GCACCATGGC 2851 CTGAAATAAC CTCTGAAAGA GGAACTTGGT TAGGTACCTT CTGAGGCGGA 2901 AAGAACCAGC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 2951 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA 3001 CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC 3051 ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA CTCCGCCCAT 3101 CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC 3151 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA 3201 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 3251 GCTTGATTCT TCTGACACAA CAGTCTCGAA CTTAAGGCTA GAGCCACCAT 3301 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA 3351 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC 3401 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC 3451 CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT 3501 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 3551 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA 3601 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG 3651 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC 3701 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG 3751 TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG 3801 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 3851 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG 3901 CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT 3951 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC 4001 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC 4051 GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC 4101 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAT 4151 GGCCGCAATA AAATATCTTT ATTTTCATTA CATCTGTGTG TTGGTTTTTT 4201 GTGTGAATCG ATAGCGATAA GGATCCTCTT TGCGCTTGCG TTTTCCCTTG 4251 TCCAGATAGC CCAGTAGCTG ACATTCATCC GGGGTCAGCA CCGTTTCTGC 4301 GGACTGGCTT TCTACGTAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 4351 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA 4401 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT 4451 TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC 4501 CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG 4551 TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC 4601 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 4651 ACGTTTTCCA ATGATGAGCA CTTTCAAAGT TCTGCTATGT GGCGCGGTAT 4701 TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT 4751 TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC 4801 GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG 4851 ATAACACTGC GGCCAACTTA CTTCTGACAA CTATCGGAGG ACCGAAGGAG 4901 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 4951 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA 5001 CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA 5051 CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA 5101 TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA 5151 TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA 5201 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 5251 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG 5301 GTGCCTCACT GATTAAGCAT TGGTAATTCG AAATGACCGA CCAAGCGACG 5351 CCCAACCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA 5401 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 5451 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC 5501 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 5551 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 5601 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 5651 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC 5701 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 5751 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA 5801 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 5851 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 5901 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 5951 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA 6001 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 6051 GCGCAGAAAA AAAGGATTTC AAGAAGATCC TTTGATCTTT TCTACGGGGT 6101 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 6151 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTATAGTCCG GAAATACAGG 6201 AACGCACGCT GGATGGCCCT TCGCTGGGAT GGTGAAACCA TGAAAAATGG 6251 CAGCTTCAGT GGATTAAGTG GGGGTAATGT GGCCTGTACC CTCTGGTTGC 6301 ATAGGTATTC ATACGGTTAA AATTTATCAG GCGCGATTGC GGCAGTTTTT 6351 CGGGTGGTTT GTTGCCATTT TTACCTGTCT GCTGCCGTGA TCGCGCTGAA 6401 CGCGTTTTAG CGGTGCGTAC AATTAAGGGA TTATGGTAAA TCCACTTACT 6451 GTCTGCCCTC GTAGCCATCG AGATAAACCG CAGTACTCCG GCCACGATGC 6501 GTCCGGCGTA GAGGATCGAG ATCT Sequence file prepared 25-June-2011.