pFC16A (HaloTag(R) 7) CMVd2 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pFC16A (HaloTag(R) 7) CMVd2 Flexi(R) Vector sequence reference points: Base pairs 4134 CMVd2 intermediate early enhancer/promoter 1-73 T7 RNA polymerase promoter (-17 to +3) 84-103 SP6 RNA polymerase promoter (-17 to +3) 108-127 SgfI region 134-141 barnase coding region 165-500 EcoICRI region 520-525 TEV protease recognition sequence 540-560 HaloTag(R) 7 coding region 570-1460 SV40 late polyadenylation signal 1594-1815 Beta-lactamase (Ampr) coding region 2076-2936 ColE1-derived plasmid origin of replication 3091-3127 Cer region (site for E. coli XerCD recombinase) 3798-4083 .. 1 GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG 51 CTGGTTTAGT GAACCGTCAG ATCACTAGAA GCTTAATACG ACTCACTATA 101 GGGTACGATT TAGGTGACAC TATAGAATAA GGAGCGATCG CCATGGAATA 151 AGTAAGGAAT CCACATGGCA CAGGTTATCA ACACGTTTGA CGGGGTTGCG 201 GATTATCTTC AGACATATCA TAAGCTACCT GATAATTACA TTACAAAATC 251 AGAAGCACAA GCCCTCGGCT GGGTGGCATC AAAAGGGAAC CTTGCAGACG 301 TCGCTCCGGG GAAAAGCATC GGCGGAGACA TCTTCTCAAA CAGGGAAGGC 351 AAACTCCCGG GCAAAAGCGG ACGAACATGG CGTGAAGCGG ATATTAACTA 401 TACATCAGGC TTCAGAAATT CAGACCGGAT TCTTTACTCA AGCGACTGGC 451 TGATTTACAA AACAACGGAC CATTATCAGA CCTTTACAAA AATCAGATAA 501 TGTTTAATGA CCCCGTGTCG AGCTCTCGAG CCAACCACTG AGGATCTGTA 551 CTTTCAGAGC GATAACGATG GATCCGAAAT CGGTACTGGC TTTCCATTCG 601 ACCCCCATTA TGTGGAAGTC CTGGGCGAGC GCATGCACTA CGTCGATGTT 651 GGTCCGCGCG ATGGCACCCC TGTGCTGTTC CTGCACGGTA ACCCGACCTC 701 CTCCTACGTG TGGCGCAACA TCATCCCGCA TGTTGCACCG ACCCATCGCT 751 GCATTGCTCC AGACCTGATC GGTATGGGCA AATCCGACAA ACCAGACCTG 801 GGTTATTTCT TCGACGACCA CGTCCGCTTC ATGGATGCCT TCATCGAAGC 851 CCTGGGTCTG GAAGAGGTCG TCCTGGTCAT TCACGACTGG GGCTCCGCTC 901 TGGGTTTCCA CTGGGCCAAG CGCAATCCAG AGCGCGTCAA AGGTATTGCA 951 TTTATGGAGT TCATCCGCCC TATCCCGACC TGGGACGAAT GGCCAGAATT 1001 TGCCCGCGAG ACCTTCCAGG CCTTCCGCAC CACCGACGTC GGCCGCAAGC 1051 TGATCATCGA TCAGAACGTT TTTATCGAGG GTACGCTGCC GATGGGTGTC 1101 GTCCGCCCGC TGACTGAAGT CGAGATGGAC CATTACCGCG AGCCGTTCCT 1151 GAATCCTGTT GACCGCGAGC CACTGTGGCG CTTCCCAAAC GAGCTGCCAA 1201 TCGCCGGTGA GCCAGCGAAC ATCGTCGCGC TGGTCGAAGA ATACATGGAC 1251 TGGCTGCACC AGTCCCCTGT CCCGAAGCTG CTGTTCTGGG GCACCCCAGG 1301 CGTTCTGATC CCACCGGCCG AAGCCGCTCG CCTGGCCAAA AGCCTGCCTA 1351 ACTGCAAGGC TGTGGACATC GGCCCGGGTC TGAATCTGCT GCAAGAAGAC 1401 AACCCGGACC TGATCGGCAG CGAGATCGCG CGCTGGCTGT CTACTCTGGA 1451 GATTTCCGGT TAATAGAATT CTAGAGTCGA CCTGCAGGCA TGCAAGCTGA 1501 TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG 1551 CTGAGCAATA ACTAGCATAA CCCCTTGGGG CGGCCGCTTC GAGCAGACAT 1601 GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA ATGCAGTGAA 1651 AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC 1701 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT 1751 GTTTCAGGTT CAGGGGGAGA TGTGGGAGGT TTTTTTAAGC AAGTAAAACC 1801 TCTACAAATG TGGTAAAATC GAATTCTAAT GGATCCTCTT TGCGCTTGCG 1851 TTTTCCCTTG TCCAGATAGC CCAGTAGCTG ACATTCATCC GGGGTCAGCA 1901 CCGTTTCTGC GGACTGGCTT TCTACGTAAT GGTTTCTTAG ACGTCAGGTG 1951 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA 2001 ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT 2051 TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG 2101 CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA 2151 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 2201 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC 2251 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTCAAAGT TCTGCTATGT 2301 GGCGCGGTAT TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG 2351 CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA 2401 AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA 2451 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CTATCGGAGG 2501 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC 2551 GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG 2601 CGTGACACCA CGATGCCTGT AGCAATGGCA ACAACGTTGC GCAAACTATT 2651 AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA 2701 TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT 2751 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 2801 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA 2851 TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC 2901 GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAATTCG AAATGACCGA 2951 CCAAGCGACG CCCAACCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 3001 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC 3051 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 3101 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 3151 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 3201 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG 3251 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 3301 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC 3351 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 3401 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 3451 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 3501 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC 3551 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 3601 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC 3651 AGCAGATTAC GCGCAGAAAA AAAGGATTTC AAGAAGATCC TTTGATCTTT 3701 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 3751 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTATAGTCCG 3801 GAAATACAGG AACGCACGCT GGATGGCCCT TCGCTGGGAT GGTGAAACCA 3851 TGAAAAATGG CAGCTTCAGT GGATTAAGTG GGGGTAATGT GGCCTGTACC 3901 CTCTGGTTGC ATAGGTATTC ATACGGTTAA AATTTATCAG GCGCGATTGC 3951 GGCAGTTTTT CGGGTGGTTT GTTGCCATTT TTACCTGTCT GCTGCCGTGA 4001 TCGCGCTGAA CGCGTTTTAG CGGTGCGTAC AATTAAGGGA TTATGGTAAA 4051 TCCACTTACT GTCTGCCCTC GTAGCCATCG AGATAAACCG CAGTACTCCG 4101 GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCT Vector sequence prepared 28-Jan-08.