pF25K ICE T7 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pF25K ICE T7 Flexi(R) Vector sequence reference points: Base pairs 3942 T7 RNA polymerase promoter 21-39 5' polyhedrin UTR 40-85 Sgf I region 86-93 Barnase coding region 117-452 Pme I region 454-461 3' polyhedrin UTR 610-983 Synthetic poly(A) signal 988-1017 T7 terminator 1018-1065 Kanamycin resistance (Kanr) coding region 1493-2287 ColE1-derived plasmid origin of replication 2456-2492 cer region (for E. coli XerCD recombinase) 3163-3448 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGA GTATTTTTAT 51 TCTTTCGTAA AAAAATTAGA AAAATAAAAT ATAAAGCGAT CGCCATGGAA 101 TAAGTAAGGA ATCCACATGG CACAGGTTAT CAACACGTTT GACGGGGTTG 151 CGGATTATCT TCAGACATAT CATAAGCTAC CTGATAATTA CATTACAAAA 201 TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA TCAAAAGGGA ACCTTGCAGA 251 CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA CATCTTCTCA AACAGGGAAG 301 GCAAACTCCC GGGCAAAAGC GGACGAACAT GGCGTGAAGC GGATATTAAC 351 TATACATCAG GCTTCAGAAA TTCAGACCGG ATTCTTTACT CAAGCGACTG 401 GCTGATTTAC AAAACAACGG ACCATTATCA GACCTTTACA AAAATCAGAT 451 AATGTTTAAA CGAATTCGGG CTCGGTACCC GGGGATCCTC TAGAGTCGAC 501 CTGCAGGCAT GCAAGCTGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT 551 GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CTAGAGTCGG 601 GCGGCTGTAA AACACGATAC ATTGTTATTA GTACATTTAT TAAGCGCTAG 651 ATTCTGTGCG TTGTTGATTT ACAGACAATT GTTGTACGTA TTTTAATAAT 701 TCATTAAATT TATAATCTTT AGGGTGGTAT GTTAGAGCGA AAATCAAATG 751 ATTTTCAGCG TCTTTATATC TGAATTTAAA TATTAAATCC TCAATAGATT 801 TGTAAAATAG GTTTCGATTA GTTTCAAACA AGGGTTGTTT TTCCGAACCG 851 ATGGCTGGAC TATCTAATGG ATTTTCGCTC AACGCCACAA AACTTGCCAA 901 ATCTTGTAGC AGCAATCTAG CTTTGTCGAT ATTCGTTTGT GTTTTGTTTT 951 GTAATAAAGG TTCGACGTCG TTCAAAATAT TATCCGCAAA AAAAAAAAAA 1001 AAAAAAAAAA AAAAAAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC 1051 TTGAGGGGTT TTTTGCTCGC GAGCGGCCGC AGGCCTCTAA ACGGGTCTTG 1101 AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGTTCGC TTGCTGTCCA 1151 TAAAACCGCC CAGTCTAGCT ATCGCCATGT AAGCCCACTG CAAGCTACCT 1201 GCTTTCTCTT TGCGCTTGCG TTTTCCCTTG TCCAGATAGC CCAGTAGCTG 1251 ACATTCATCC GGGGTCAGCA CCGTTTCTGC GGACTGGCTT TCTACGTGTT 1301 CCGCTTCCTT TAGCAGCCCT TGCGCCCTGA GTGCTTGCGG CAGCGTGAGC 1351 TTCAAAAGAA TTGCCAGCTG GGGCGCCCTC TGGTAAGGTT GGGAAGCCCT 1401 GCAAAGTAAA CTGGATGGCT TTCTTGCCGC CAAGGATCTG ATGGCGCAGG 1451 GGATCAAGAT CTGATCAAGA GACAGGATGA CGGTCGTTTC GCATGCTTGA 1501 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT 1551 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 1601 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT 1651 GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC 1701 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 1751 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT 1801 GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA 1851 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 1901 GCGAAACATC GCATCGAGCG AGCACGCACT CGGATGGAAG CCGGTCTTGT 1951 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC 2001 TGTTCGCCAG GCTCAAGGCG CGTATGCCGG ATGGTGAGGA TCTCGTCGTG 2051 ACTCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT 2101 TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG 2151 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 2201 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG 2251 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG 2301 GTTCGAAATG ACCGACCAAG CGACGCCCAA CCGGTATCAG CTCACTCAAA 2351 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA 2401 TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG 2451 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 2501 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 2551 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 2601 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 2651 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA 2701 AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA 2751 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 2801 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 2851 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG 2901 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 2951 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT 3001 TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATTTCAAGAA 3051 GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 3101 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 3151 TCCTTTTATA GTCCGGAAAT ACAGGAACGC ACGCTGGATG GCCCTTCGCT 3201 GGGATGGTGA AACCATGAAA AATGGCAGCT TCAGTGGATT AAGTGGGGGT 3251 AATGTGGCCT GTACCCTCTG GTTGCATAGG TATTCATACG GTTAAAATTT 3301 ATCAGGCGCG ATTGCGGCAG TTTTTCGGGT GGTTTGTTGC CATTTTTACC 3351 TGTCTGCTGC CGTGATCGCG CTGAACGCGT TTTAGCGGTG CGTACAATTA 3401 AGGGATTATG GTAAATCCAC TTACTGTCTG CCCTCGTAGC CATCGAGATA 3451 AACCGCAGTA CTCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTT 3501 TTCAGCCTGA TACAGATTAA ATCAGAACGC AGAAGCGGTC TGATAAAACA 3551 GAATTTGCCT GGCGGCAGTA GCGCGGTGGT CCCACCTGAC CCCATGCCGA 3601 ACTCAGAAGT GAAACGCCGT AGCGCCGATG GTAGTGTGGG GTCTCCCCAT 3651 GCGAGAGTAG GGAACTGCCA GGCATCAAAT AAAACGAAAG GCTCAGTCGA 3701 AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA CGCTCTCCTG 3751 AGTAGGACAA ATCCGCCGGG AGCGGATTTG AACGTTGCGA AGCAACGGCC 3801 CGGAGGGTGG CGGGCAGGAC GCCCGCCATA AACTGCCAGG CATCAAATTA 3851 AGCAGAAGGC CATCCTGACG GATGGCCTTT TTGCGTTTCT ACAAACTCTT 3901 TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC AT Sequence file prepared 11-Aug-08.