pF1K T7 Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pf1K T7 Flexi(R) Vector sequence reference points: Base pairs 3450 T7 RNA polymerase promoter (-17 to +3) 21-40 Sgf I site 100-107 Barnase coding region 109-444 Pme I site 446-453 T7 terminator 573-620 Kanamycin resistance coding region 1001-1795 ColE1-derived plasmid origin of replication 1964-2000 cer site (site for E. coli XerCD recombinase) 2671-2956 rrnB transcription terminator 3007-3408 .. 1 GGATCTCGAT CCCGCGAAAT TAATACGACT CACTATAGGG GAATTGTGAG 51 CGGATAACAA TTCCCCACTA GTAATAATTT TCTTTAACTT TAGTAAGGAG 101 CGATCGCCAT GGCACAGGTT ATCAACACGT TTGACGGGGT TGCGGATTAT 151 CTTCAGACAT ATCATAAGCT ACCTGATAAT TACATTACAA AATCAGAAGC 201 ACAAGCCCTC GGCTGGGTGG CATCAAAAGG GAACCTTGCA GACGTCGCTC 251 CGGGGAAAAG CATCGGCGGA GACATCTTCT CAAACAGGGA AGGCAAACTC 301 CAGGGCAAAA GCGGACGAAC ATGGCGTGAA GCGGATATTA ACTATACATC 351 AGGCTTCAGA AATTCAGACC GGATTCTTTA CTCAAGCGAC TGGCTGATTT 401 ACAAAACAAC GGACCATTAT CAGACCTTTA CAAAAATCAG ATAATGTTTA 451 AACGAATTCG AGCTCGGTAC CCGGGGATCC TCTAGAGTCG ACCTGCAGGC 501 ATGCAAGCTG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC 551 TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC 601 GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGTTCGCTT 651 GCTGTCCATA AAACCGCCCA GTCTAGCTAT CGCCATGTAA GCCCACTGCA 701 AGCTACCTGC TTTCTCTTTG CGCTTGCGTT TTCCCTTGTC CAGATAGCCC 751 AGTAGCTGAC ATTCATCCGG GGTCAGCACC GTTTCTGCGG ACTGGCTTTC 801 TACGTGTTCC GCTTCCTTTA GCAGCCCTTG CGCCCTGAGT GCTTGCGGCA 851 GCGTGAGCTT CAAAAGAATT GCCAGCTGGG GCGCCCTCTG GTAAGGTTGG 901 GAAGCCCTGC AAAGTAAACT GGATGGCTTT CTTGCCGCCA AGGATCTGAT 951 GGCGCAGGGG ATCAAGATCT GATCAAGAGA CAGGATGACG GTCGTTTCGC 1001 ATGCTTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 1051 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG 1101 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG 1151 ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT 1201 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG 1251 TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 1301 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 1351 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG 1401 ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGCACTCG GATGGAAGCC 1451 GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC 1501 AGCCGAACTG TTCGCCAGGC TCAAGGCGCG TATGCCGGAT GGTGAGGATC 1551 TCGTCGTGAC TCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 1601 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 1651 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG 1701 GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT 1751 TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG 1801 ACTCTGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC GGTATCAGCT 1851 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 1901 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 1951 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 2001 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 2051 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA 2101 CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG 2151 GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 2201 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 2251 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 2301 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 2351 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA 2401 CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC 2451 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 2501 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 2551 TTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 2601 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 2651 CACCTAGATC CTTTTATAGT CCGGAAATAC AGGAACGCAC GCTGGATGGC 2701 CCTTCGCTGG GATGGTGAAA CCATGAAAAA TGGCAGCTTC AGTGGATTAA 2751 GTGGGGGTAA TGTGGCCTGT ACCCTCTGGT TGCATAGGTA TTCATACGGT 2801 TAAAATTTAT CAGGCGCGAT TGCGGCAGTT TTTCGGGTGG TTTGTTGCCA 2851 TTTTTACCTG TCTGCTGCCG TGATCGCGCT GAACGCGTTT TAGCGGTGCG 2901 TACAATTAAG GGATTATGGT AAATCCACTT ACTGTCTGCC CTCGTAGCCA 2951 TCGAGATAAA CCGCAGTACT CCGGCCACGA TGCGTCCGGC GTAGAGGATC 3001 GAGATCTTTT CAGCCTGATA CAGATTAAAT CAGAACGCAG AAGCGGTCTG 3051 ATAAAACAGA ATTTGCCTGG CGGCAGTAGC GCGGTGGTCC CACCTGACCC 3101 CATGCCGAAC TCAGAAGTGA AACGCCGTAG CGCCGATGGT AGTGTGGGGT 3151 CTCCCCATGC GAGAGTAGGG AACTGCCAGG CATCAAATAA AACGAAAGGC 3201 TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG 3251 CTCTCCTGAG TAGGACAAAT CCGCCGGGAG CGGATTTGAA CGTTGCGAAG 3301 CAACGGCCCG GAGGGTGGCG GGCAGGACGC CCGCCATAAA CTGCCAGGCA 3351 TCAAATTAAG CAGAAGGCCA TCCTGACGGA TGGCCTTTTT GCGTTTCTAC 3401 AAACTCTTTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT Sequence file prepared 30-Nov-04.