pF12A RM Flexi(R) Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pF12A RM Flexi(R) Vector sequence reference points: Base pairs 3470 12X lambda operator 16-309 Minimal CMV promoter 322-434 SgfI site 446-453 Barnase coding region 477-812 PmeI site 814-821 SV40 late poly(A) signal 973-1194 Beta-lactamase (Ampr) coding region 1455-2315 ColE1-derived origin of replication 2470-2506 cer site (site for E. coli XerCD recombinase) 3177-3462 .. 1 AGTACTCCGC TCGAGTTTAC CTCTGGCGGT GATAGTCGAG TTTACCTCTG 51 GCGGTGATAG TCGAGTTTAC CTCTGGCGGT GATAGTCGAG TTTACCTCTG 101 GCGGTGATAG TCGAGTTTAC CTCTGGCGGT GATAGTCGAG TTTACCTCTG 151 GCGGTGATAG TCGAGTTTAC CTCTGGCGGT GATAGTCGAG TTTACCTCTG 201 GCGGTGATAG TCGAGTTTAC CTCTGGCGGT GATAGTCGAG TTTACCTCTG 251 GCGGTGATAG TCGAGTTTAC CTCTGGCGGT GATAGTCGAG TTTACCTCTG 301 GCGGTGATAG TCGACTCTAG ATAGGCGTGT ACGGTGGGAG GCCTATATAA 351 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC CTGGAGACGC CATCCACGCT 401 GTTTTGACCT CCATAGAAGA CACCGGGACC GATCAACCTA AGCTTGCGAT 451 CGCCATGGAA TAAGTAAGGA ATCCACATGG CACAGGTTAT CAACACGTTT 501 GACGGGGTTG CGGATTATCT TCAGACATAT CATAAGCTAC CTGATAATTA 551 CATTACAAAA TCAGAAGCAC AAGCCCTCGG CTGGGTGGCA TCAAAAGGGA 601 ACCTTGCAGA CGTCGCTCCG GGGAAAAGCA TCGGCGGAGA CATCTTCTCA 651 AACAGGGAAG GCAAACTCCC GGGCAAAAGC GGACGAACAT GGCGTGAAGC 701 GGATATTAAC TATACATCAG GCTTCAGAAA TTCAGACCGG ATTCTTTACT 751 CAAGCGACTG GCTGATTTAC AAAACAACGG ACCATTATCA GACCTTTACA 801 AAAATCAGAT AATGTTTAAA CGAATTCGGG CTCGGTACCC GGGGATCCTC 851 TAGAGTCGAC CTGCAGGCAT GCAAGCTGAT CCGGCTGCTA ACAAAGCCCG 901 AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC 951 CCCTTGGGGC GGCCGCTTCG AGCAGACATG ATAAGATACA TTGATGAGTT 1001 TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA 1051 TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA 1101 GTTAACAACA ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGAT 1151 GTGGGAGGTT TTTTTAAGCA AGTAAAACCT CTACAAATGT GGTAAAATCG 1201 AATTCTAATG GATCCTCTTT GCGCTTGCGT TTTCCCTTGT CCAGATAGCC 1251 CAGTAGCTGA CATTCATCCG GGGTCAGCAC CGTTTCTGCG GACTGGCTTT 1301 CTACGTAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC 1351 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 1401 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA 1451 GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG 1501 CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA 1551 GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT 1601 CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 1651 TGATGAGCAC TTTCAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT 1701 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA 1751 CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA 1801 CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG 1851 GCCAACTTAC TTCTGACAAC TATCGGAGGA CCGAAGGAGC TAACCGCTTT 1901 TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 1951 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA 2001 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT 2051 AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG 2101 GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA 2151 TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC 2201 AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG 2251 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG 2301 ATTAAGCATT GGTAATTCGA AATGACCGAC CAAGCGACGC CCAACCGGTA 2351 TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 2401 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 2451 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG 2501 CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 2551 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG 2601 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA 2651 AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 2701 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 2751 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA 2801 CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 2851 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG 2901 GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT 2951 ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 3001 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 3051 AAGGATTTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG 3101 TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 3151 GATCTTCACC TAGATCCTTT TATAGTCCGG AAATACAGGA ACGCACGCTG 3201 GATGGCCCTT CGCTGGGATG GTGAAACCAT GAAAAATGGC AGCTTCAGTG 3251 GATTAAGTGG GGGTAATGTG GCCTGTACCC TCTGGTTGCA TAGGTATTCA 3301 TACGGTTAAA ATTTATCAGG CGCGATTGCG GCAGTTTTTC GGGTGGTTTG 3351 TTGCCATTTT TACCTGTCTG CTGCCGTGAT CGCGCTGAAC GCGTTTTAGC 3401 GGTGCGTACA ATTAAGGGAT TATGGTAAAT CCACTTACTG TCTGCCCTCG 3451 TAGCCATCGA GATAAACCGC Sequence file prepared 2-Mar-07.