pReg neo Vector This vector can be obtained from Promega Corporation, Madison, WI. Call one of the following numbers for order or technical information. Order or Technical 1-800-356-9526 Outside U.S. 608-274-4330 pReg neo Vector sequence reference points: Base pairs 6802 SV40 early promoter 141-433 6X lambda operator 444-587 Minimal CMV promoter 600-659 Rep-GyrB-AD 770-2443 Lambda repressor (DNA binding domain) 770-1085 GyrB (amino terminal domain) 1086-1758 p65 Activation Domain (AD) 1759-2443 SV40 late polyadenylation signal 2497-2718 SV40 early enhancer/promoter region 3330-3748 Neomycin phosphotransferase (Neor) coding region 3793-4587 Synthetic polyadenylation signal 4651-4699 Beta-lactamase (Ampr) coding region 5110-5970 ColE1-derived plasmid origin of replication 6695-6732 .. 1 TGGCTTAACT ATGCGACCGG TGCCTCCATA TGGACATATT GTCGTTAGAA 51 CGCGGCTACA ATTAATACAT AACCTTATGT ATCATACACA TACGATTTAG 101 GTGACACTAT AGAACTCGAT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA 151 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT 201 AGTCAGCAAG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG 251 CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA 301 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA 351 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT 401 ATTCCAGAAG TAGTGAAGAG GCTTTTTTGG AGGAGATCTC GAGTTTACCT 451 CTGGCGGTGA TAGTCGAGTT TACCTCTGGC GGTGATAGTC GAGTTTACCT 501 CTGGCGGTGA TAGTCGAGTT TACCTCTGGC GGTGATAGTC GAGTTTACCT 551 CTGGCGGTGA TAGTCGAGTT TACCTCTGGC GGTGATAGTC GACTCTAGAT 601 AGGCGTGTAC GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG 651 TCAGATCGCC TGGAGACGCC ATCCACGCTG TTTTGACCTC CATAGAAGAC 701 ACCGGGACCG ATCCAGCCTA AGCTTAATAC GACTCACTAT AGGGAGACCC 751 AAGCTGGGAA GCTTCCACCA TGAGCACAAA AAAGAAACCA TTAACACAAG 801 AGCAGCTTGA GGACGCACGT CGCCTTAAAG CAATTTATGA AAAAAAGAAA 851 AATGAACTTG GCTTATCCCA GGAATCTGTC GCAGACAAGA TGGGGATGGG 901 GCAGTCAGGC GTTGGTGCTT TATTTAATGG CATCAATGCA TTAAATGCTT 951 ATAACGCCGC ATTGCTTGCA AAAATTCTCA AAGTTAGCGT TGAAGAATTT 1001 AGCCCTTCAA TCGCCAGAGA AATCTACGAG ATGTATGAAG CGGTAGGGAT 1051 GCAGCCGTCA CTTAGAAGTG AGTATGAGTA CCCTGTTTTT TCTCATGTTC 1101 AGGCAGGGAT GTTCTCACCT AAGCTTAGAA CCTTTACCAA AGGTGATGCG 1151 GAGAGATGGG TAGATATCTC GAATTCTTAT GACTCCTCCA GTATCAAAGT 1201 CCTGAAAGGG CTGGATGCGG TGCGTAAGCG CCCGGGTATG TATATCGGCG 1251 ACACGGATGA CGGCACCGGT CTGCACCACA TGGTATTCGA GGTGGTAGAT 1301 AACGCTATCG ACGAAGCGCT CGCGGGTCAC TGTAAAGAAA TTATCGTCAC 1351 CATTCACGCC GATAACTCTG TCTCTGTACA GGATGACGGG CGCGGCATTC 1401 CGACCGGTAT TCACCCGGAA GAGGGCGTAT CGGCGGCGGA AGTGATCATG 1451 ACCGTTCTGC ACGCAGGCGG TAAATTTGAC GATAACTCCT ATAAAGTGTC 1501 CGGCGGTCTG CACGGCGTTG GTGTTTCGGT AGTAAACGCC CTGTCGCAAA 1551 AACTGGAGCT GGTTATCCAG CGCGAGGGTA AAATTCACCG TCAGATCTAC 1601 GAACACGGTG TACCGCAGGC CCCGCTGGCG GTTACCGGCG AGACTGAAAA 1651 AACCGGCACC ATGGTGCGTT TCTGGCCCAG CCTCGAAACC TTCACCAATG 1701 TGACCGAGTT CGAATATGAA ATTCTGGCGA AACGTCTGCG TGAGTTGTCG 1751 TTCCTCAACT CCGGCGTTTC CATTCGTCTG CGCGACAAGC GCGACGGCAA 1801 AGAAGACCAC TTCCACTATG AAGGCGGCCC ATGTATGGGC CCTAAAAAGA 1851 AGCGTAAAGT CGCCATCGAT CAGCTCACCA TGGTGTTTCC TTCTGGGCAG 1901 ATCTCAAACC AGGCCCTGGC CTTAGCACCG TCCTCTGCCC CAGTCCTTGC 1951 CCAGACCATG GTCCCTTCCT CAGCCATGGT ACCTCTGGCT CAGCCCCCAG 2001 CTCCTGCCCC AGTTCTAACC CCGGGTCCTC CCCAGTCCCT GTCTGCACCT 2051 GTTCCAAAGA GCACCCAGGC TGGGGAAGGC ACGCTGTCGG AAGCCCTGCT 2101 GCACCTGCAG TTTGATGCTG ATGAAGACTT GGGGGCCTTG CTTGGCAACA 2151 GCACAGACCC AGGAGTGTTC ACAGACCTGG CATCTGTGGA CAACTCAGAG 2201 TTTCAGCAGC TCCTGAACCA GGGTGTGTCC ATGTCTCACT CCACAGCTGA 2251 GCCCATGCTG ATGGAGTACC CTGAAGCTAT AACTCGCCTG GTGACAGGGT 2301 CCCAGAGGCC CCCTGACCCA GCTCCCACAC CCCTGGGGAC CTCGGGGCTT 2351 CCCAATGGTC TCTCCGGAGA TGAAGACTTC TCCTCCATTG CGGACATGGA 2401 CTTCTCTGCT CTGCTGAGTC AGATCAGCTC CAGCGGCCAA TAATCTAGAG 2451 TCGACCCGGG CGGCCGCTTC CCTTTAGTGA GGGTTAATGC TTCGAGCAGA 2501 CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT 2551 GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA 2601 ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT 2651 TATGTTTCAG GTTCAGGGGG AGATGTGGGA GGTTTTTTAA AGCAAGTAAA 2701 ACCTCTACAA ATGTGGTAAA ATCCGATAAG GATCGATCCG GGCTGGCGTA 2751 ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG 2801 AATGGCGAAT GGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 2851 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC 2901 TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 2951 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 3001 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG 3051 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT 3101 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 3151 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG 3201 GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA 3251 TATTAACGCT TACAATTTCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT 3301 GCGGTATTTC ACACCGCATA CGCGGATCTG CGCAGCACCA TGGCCTGAAA 3351 TAACCTCTGA AAGAGGAACT TGGTTAGGTA CCTTCTGAGG CGGAAAGAAC 3401 CAGCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC 3451 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT 3501 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT 3551 CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC 3601 CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT 3651 TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CGGCCTCTGA GCTATTCCAG 3701 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTTGA 3751 TTCTTCTGAC ACAACAGTCT CGAACTTAAG GCTAGAGCCA CCATGATTGA 3801 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT 3851 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG 3901 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT 3951 GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC 4001 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA 4051 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT 4101 GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA 4151 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA 4201 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT 4251 CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC 4301 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG 4351 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT 4401 TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG 4451 ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG 4501 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG 4551 CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG 4601 GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGATGGCCGC 4651 AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT TTTTGTGTGA 4701 ATCGATAGCG ATAAGGATCC GCGTATGGTG CACTCTCAGT ACAATCTGCT 4751 CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC 4801 GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT 4851 GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA 4901 AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 4951 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA 5001 TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 5051 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA 5101 AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT 5151 TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG 5201 TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 5251 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT 5301 TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 5351 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG 5401 AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG 5451 CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA 5501 CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 5551 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA 5601 ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 5651 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT 5701 ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT 5751 TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG 5801 ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 5851 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG 5901 TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 5951 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT 6001 TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT 6051 CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC 6101 ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 6151 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC 6201 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 6251 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG 6301 CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT 6351 CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT 6401 GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 6451 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC 6501 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC 6551 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA 6601 ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA 6651 TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT 6701 GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 6751 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGG CTCGACAGAT 6801 CT Sequence file revised 1-Nov-07.